Poxvirus-derived promoter, and vector comprising same
The present invention provides a poxvirus-derived promoter, a vector comprising the same, a method for expressing a transgene using the promoter, and use of the vector in the prevention or treatment of a disease. A promoter according to the present invention can be used for induction of strong expression of a transgene.
1. A recombinant nucleic acid molecule consisting of two or three different polynucleotides selected from the group consisting of the polynucleotides of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3,
wherein the two or three polynucleotides are consecutively linked in 5′ to 3′ direction in a way that 3′-end of an immediate preceding polynucleotide is linked to 5′-end of a next polynucleotide.
2. The recombinant nucleic acid molecule according to claim 1 , consisting of:
the polynucleotide of SEQ ID NO: 1; and
one or two polynucleotides selected from the group consisting of the polynucleotides of SEQ ID NO: 2 and SEQ ID NO: 3.
3. The recombinant nucleic acid molecule according to claim 2 , wherein the one or two polynucleotides selected from the group consisting of the polynucleotides of SEQ ID NO: 2 and SEQ ID NO: 3 is/are linked to the 3′-end of the polynucleotide of SEQ ID NO: 1 in a 5′ to 3′ direction.
4. A recombinant nucleic acid construct consisting of (a) the recombinant nucleic acid molecule according to claim 2 and (b) a restriction enzyme recognition site at the 5′-end and/or 3′-end of the recombinant nucleic acid molecule.
5. The recombinant nucleic acid molecule according to claim 1 , comprising the nucleotide sequence of SEQ ID NO: 9 or SEQ ID NO: 10.
6. A recombinant nucleic acid construct consisting of (a) the recombinant nucleic acid molecule according to claim 1 and (b) a nucleotide encoding a target protein, so as to induce expression of the target protein-encoding nucleotide sequence in a host cell.
7. The recombinant nucleic acid construct according to claim 6 , wherein the host cell is a mammalian cell and the target protein is expressed in cytoplasm of the mammalian cell.
8. A vector comprising the recombinant nucleic acid molecule according to claim 1 .
9. The vector according to claim 8 , which is a virus.
10. The vector according to claim 9 , which is derived from a virus of a poxviridae.
11. The vector according to claim 10 , wherein the virus of the poxviridae is selected from the group consisting of the viruses of orthopoxvirus, avipoxvirus, parapoxvirus, capripoxvirus, and suipoxvirus genuses.
12. The vector according to claim 10 , wherein the poxvirus is vaccinia virus.
13. A vector comprising the recombinant nucleic acid construct according to claim 6 .
14. The vector according to claim 13 , wherein the target protein is a tumor antigen, an immune response-inducing factor, a tumor growth-inhibitory factor, an apoptosis-inducing factor, or a factor which can aid in enhancing an activity of a virus in a tumor tissue.
15. A host transformed with a vector comprising the recombinant nucleic acid molecule according to claim 1 .
16. The host according to claim 15 , which is a microorganism, a mammal, a mammalian cell, or a cell line derived from a mammal.
17. A vector comprising the recombinant nucleic acid molecule according to claim 5 .
18. A vector comprising the recombinant nucleic acid construct according to claim 4 .
19. A vector comprising the recombinant nucleic acid molecule according to claim 3 .
20. A vector comprising the recombinant nucleic acid molecule according to claim 2 .