Method for detecting or quantifying carbon black and/or black carbon particles
A method for detecting the presence of or quantification of carbon black and/or black carbon in a sample or carrier medium. The method includes providing the sample or carrier medium without labelling or pre-treatment of the carbon black and/or black carbon particles; illuminating the sample or carrier medium at a temperature below 90° C. by a pulsed light with a pulse duration below 500 femtoseconds, a repetition rate above 1 MHz with an average power below 20 mW, and a wavelength of a femtosecond laser pulse ranging from 700 to 1200 nm, to generate non-incandescence related light emission from the carbon black and/or black carbon particles; and analysis of the light emission.
1. A method for quantification of carbon black and/or black carbon in a sample or carrier medium, the method comprising:
providing the sample or carrier medium without labelling of the carbon black and/or black carbon particles and without pre-treatment of the carbon black and/or black carbon particles;
illuminating the sample or carrier medium at a temperature below 90° C. by a pulsed light with a pulse duration below 500 femtoseconds, a repetition rate above 1 MHz with an average power below 20 mW, and a wavelength of a femtosecond laser pulse ranging from 700 to 1200 nm, to generate non-incandescence related light emission from the carbon black and/or black carbon particles; and
analysis of the light emission to quantify the carbon black and/or black carbon in the sample or carrier medium.
2. The method according to claim 1 , wherein the illumination is performed with a femtosecond laser focused on the sample using a stationary or scanning beam delivery.
3. The method according to claim 1 , wherein the average power of the pulsed light is between 4 and 5 mW.
4. The method according to claim 1 , wherein the sample or carrier medium comprises a material or compound with autofluorescence properties.
5. The method according to claim 1 , wherein the sample or carrier medium comprises a compound selected from the group consisting of mitochondria, lysosomes, flavins, extracellular matrix, collagen, elastin, NAD(P)H, chlorophyll, retinol, cholecalciferol, folic acid, pyridoxine, tyrosine, dityrosine, excimer-like aggregate, glycation adduct, indolamine, lipofuscin, polyphenol, tryptophan, and melanin, or a combination thereof.
6. The method according to claim 1 , wherein the intensity and/or wavelength of the pulsed light is varied until emission from the carbon black and/or black carbon particles is distinguishable from emission noise from the sample.
7. The method according to claim 1 , wherein the pulsed light intensity and/or wavelength is modulated to reach near infrared emission.
8. The method according to claim 7 , wherein shorter emission wavelengths than the near infrared emission are filtered out by a wave length filter to distinguish carbon black and/or black carbon particles emission from background noise emission.
9. The method according to claim 1 , wherein the sample or carrier medium is illuminated at a temperature between 1° C. and 80° C.
10. The method according to claim 1 wherein the sample or carrier medium is illuminated at a temperature between 10° C. and 50° C.
11. The method according to claim 1 , wherein the sample is blood or urine.
12. The method according to claim 1 , wherein the sample is a liquid sample, cell sample or tissue sample of biological origin, wherein the cells in the cell sample or tissue sample have an intracellular water content greater than 40%.
13. The method according to claim 1 , wherein the method comprises quantification of black carbon particles without labelling of the black carbon particles and without pre-treatment of the black carbon particles.
14. The method according to claim 13 , wherein the black carbon particles are aggregated black carbon particles.
15. The method according to claim 1 , wherein the sample or carrier medium comprises carbon black or black carbon particles from environmental or industrial pollution.