Method for extracting pea proteins
The present invention relates to a method for extracting and purifying pea proteins, comprising the steps of (a) providing an aqueous composition comprising pea proteins; (b) isolating said pea proteins from said aqueous composition comprising pea proteins; (c) obtaining said isolated pea proteins as an aqueous slurry having a pH ranging from 4.0 to 5.8; and (d) subjecting said aqueous slurry having a pH ranging from 4.0 to 5.8 to a temperature of at least 75° C. Also described herein are pea protein compositions and are food or feed products comprising said pea protein compositions.
1. A method for extracting pea proteins, comprising in the indicated order the steps of:
(a) providing an aqueous composition comprising pea proteins, obtained by fractionation of milled peas;
(b) isolating said pea proteins from said aqueous composition comprising pea proteins by isoelectric precipitation and separation of said pea proteins;
(c) obtaining said isolated pea proteins as an aqueous slurry by maintaining or adjusting the pH ranging from 4.0 to 5.8, comprising a dry matter content of 10 to 40 wt % and comprising at least 60 wt. % of protein based on the total dry matter of the aqueous slurry; and
(d) subjecting said aqueous slurry having a pH ranging from 4.0 to 5.8 to a temperature ranging from 115° C. to 210° C. for a time ranging from 15 s to 0.01 s; at a temperature ranging from 95° C. to 115° C. for a time ranging from 5 min to 15 s; at a temperature ranging from 75° C. to 95° C. for a time ranging from 15 min to 5 min; at a temperature ranging from 75° C. to 110° C. for a time ranging from 10 min to 2 min; at a temperature ranging from 80° C. to 100° C. for a time ranging from 8 min to 5 min; or at a temperature ranging from 130° C. to 150° C. for a time ranging from 8 s to 1 s;
wherein said pea proteins have a nitrogen solubility index at pH 7.0 of at most 15%, as measured on an aqueous composition comprising 3 wt. % of said pea protein composition based on the total weight of the aqueous composition.
2. The method according to claim 1 , wherein isolating pea proteins from said aqueous composition of step (b), comprises concentrating said pea proteins.
3. The method according to claim 1 , wherein step (b) comprises adjusting the pH of said aqueous composition comprising pea proteins to a value ranging from 4.0 to 5.8, or from 4.5 to 5.5.
4. The method according to claim 1 , wherein step (c) comprises adjusting or maintaining the pH of the aqueous slurry to a value ranging from 4.5 to 5.5.
5. The method according to claim 1 , wherein said aqueous composition comprising pea proteins in step (a) has a pH of at least 6, or ranging from 6.0 to 9.0, or ranging from 6.5 to 8.5.
6. The method according to claim 1 , wherein prior to step (a) an aqueous composition comprising peas is subjected to fermentation.
7. The method according to claim 6 , wherein said fermentation is performed in the presence of one or more Lactobacillus sp.
8. The method according to claim 1 , wherein prior to or during step (b) said aqueous composition comprising pea proteins, or said pea proteins are subjected to a heat treatment.
9. Pea proteins produced by the method according to claim 1 .
10. Pea protein composition, comprising at least 60 wt. % of protein based on the total dry matter of the composition, wherein said pea protein composition has a nitrogen solubility index at pH 7.0 of at most 15%, as measured on a aqueous composition comprising 3 wt. % of said pea protein composition based on the total weight of the aqueous composition.
11. Pea protein composition according to claim 10 , wherein said composition has a pH ranging from 4.0 to 5.8, as measured at room temperature on 10 g of pea protein composition suspended in 90 g of water.
12. The method according to claim 8 , wherein the heat treatment is pasteurization.
13. The method according to claim 6 , wherein said fermentation is in the presence of lactic acid bacteria.