Composition and solution with controlled calcium ion level, and related method and use for reperfusion
A solution comprises a preservation mixture comprising a calcium ion source; and a buffer for maintaining a pH of the solution. The molar concentration of calcium ion (Ca 2+ ) in the solution is from 0.18 to 0.26 mmol/L, and the pH is lower than 7.4 and higher than 6.6. A composition for preparing the solution may comprise adenosine, lidocaine, and a calcium source, wherein the molar ratio of adenosine:calcium is from 0.3:0.26 to 0.45:0.18, and the molar ratio of lidocaine:calcium is from 0.04:0.26 to 0.09:0.18. A donor heart may be reperfused with the solution. The solution may be used for reperfusion of a donor heart, such as at a temperature from about 25 to about 37° C. The donor may be a donor after circulatory death.
1. A composition for preparing a solution, the composition comprising:
adenosine;
lidocaine; and
a calcium source,
wherein the molar ratio of adenosine:calcium in the composition is from 0.3:0.26 to 0.45:0.18, and the molar ratio of lidocaine:calcium in the composition is from 0.04:0.26 to 0.09:0.18,
wherein the solution comprises a cardioplegia mixture and a buffer for maintaining a pH of the solution, the cardioplegia mixture comprises 0.3 to 0.45 mmol/L of adenosine, 0.04 to 0.09 mmol/L of lidocaine, and 0.18 to 0.26 mmol/L of calcium ion (Ca 2+ ), and the pH is lower than 7.4 and higher than 6.6.
2. The composition of claim 1 , wherein the molar ratio of adenosine:calcium is 0.4:0.22, and the molar ratio of lidocaine:calcium is 0.05:0.22.
3. The composition of claim 1 , further comprising a sodium source, a potassium source and a magnesium source, wherein the molar ratio of calcium:sodium is from 0.26:130 to 0.18:160, the molar ratio of calcium:potassium is from 0.26:4 to 0.18 to 7, and the molar ratio of calcium:magnesium is from 0.26:11 to 0.18:15.
4. The composition of claim 1 , wherein the molar concentration of calcium ion (Ca 2+ ) in the solution is 0.22 mmol/L.
5. The composition of claim 1 , wherein the pH is from 6.8 to 7.0.
6. The composition of claim 1 , wherein the pH is 6.9.
7. The composition of claim 1 , further comprising a magnesium ion source.
8. The composition of claim 7 , wherein the solution comprises 11 to 15 mmol/L of Mg 2+ .
9. The composition of claim 1 , further comprising a sodium ion source and a potassium ion source.
10. The composition of claim 9 , wherein the solution comprises about 130 to about 160 mmol/L of Na + and 4 to 7 mmol/L of K + .
11. The composition of claim 1 , further comprising chloride, an osmotic buffer and a reducing agent.
12. The composition of claim 11 , further comprising 70 to 180 mmol/L chloride, 8 to 12.5 mmol/L of glucose, 7.5 to 12.5 IU/L of insulin, 100 to 140 mmol/L of D-mannitol, 0.75 to 1.25 mmol/L of pyruvate, and 2.5 to 3.5 mmol/L of reduced glutathione.
13. The composition of claim 1 , wherein the composition is oxygenated.
14. The composition of claim 1 , wherein the solution comprises:
0.3 to 0.45 mmol/L of adenosine;
0.04 to 0.09 mmol/L of lidocaine;
8 to 12.5 mmol/L of glucose;
110 to 130 mmol/L of NaCl;
4 to 7 mmol/L of KCl;
16 to 24 mmol/L of NaHCO 3 ;
0.9 to 1.4 mmol/L of NaH 2 PO 4 ;
0.18 to 0.26 mmol/L of CaCl 2 ;
11 to 15 mmol/L of MgCl 2 ;
7.5 to 12.5 IU/L of insulin;
100 to 140 mmol/L of D-mannitol;
0.75 to 1.25 mmol/L of pyruvate; and
2.5 to 3.5 mmol/L of reduced glutathione.
15. The composition of claim 1 , wherein the solution comprises:
0.4 mmol/L of adenosine;
0.05 mmol/L of lidocaine;
10 mmol/L of glucose;
123.8 mmol/L of NaCl;
5.9 mmol/L of KCl;
20 mmol/L of NaHCO 3 ;
1.2 mmol/L of NaH 2 PO 4 ;
0.22 mmol/L of CaCl 2 ;
13 mmol/L of MgCl 2 ;
10 IU/L of insulin;
120 mmol/L of D-mannitol;
1 mmol/L of pyruvate; and
3 mmol/L of reduced glutathione.