Reagents and methods for treating bacterial infection
Disclosed herein are α-sheet peptides and their use for treating a bacterial infection and/or limiting bacterial biofilm formation.
1. A method for treating a bacterial infection, comprising administering to a subject with a bacterial infection an amount of an α-sheet peptide effective to treat the bacterial infection.
2. The method of claim 1 , wherein the bacterial infection comprises a bacterial biofilm, and wherein the treating comprises disruption of the biofilm.
3. The method of claim 1 , wherein the bacterial infection comprises an Escherichia coli , a Pseudomonas aeruginosa , or a Staphylococcus aureus bacterial infection.
4. The method of claim 1 , wherein the bacterial infection comprises a drug or multi-drug resistant bacterial infection.
5. The method of claim 1 , wherein the bacterial infection is contracted during hospitalization.
6. The method of claim 1 , wherein the a-sheet peptide comprises a peptide selected from the group consisting of:
AP90
SEQ ID NO: 1
RGEmNlSwMNEYSGWtMnLkMGR;
AP401
SEQ ID NO: 2
rGeMnLsWmneysGwTmNlKmGr;
AP407
SEQ ID NO: 3
RGEmNlCwMNEYSGWcMnLkMGR;
AP193
SEQ ID NO: 4
RGEmNyFwMNEYYGWtMnCkMGR;
and
AP5
SEQ ID NO: 8
RGNwNeSkMNEYSGWmLmLtMGR;
wherein residues in lower-case are D amino acids, residues in upper case are L amino acids, and G residues are achiral.
7. A method for limiting development of bacterial biofilm, comprising administering to a subject at risk of a bacterial infection comprising biofilm formation with an amount of an α-sheet peptide effective to limit development of the bacterial biofilm, wherein the subject is undergoing a surgical procedure.
8. The method of claim 7 , wherein the surgical procedure comprises placement of a medical device in the subject.
9. The method of claim 8 , wherein the medical device is selected from the group consisting of prosthetic heart valves, cardiac pacemakers, cerebrospinal fluid shunts, urinary catheters, intravascular catheters, ocular prostheses, prosthetic joints, orthopedic implants, titanium-containing implants, polystyrene-containing implants, and intrauterine contraceptive devices.
10. The method of claim 9 , wherein the a-sheet peptide is placed on the medical device prior to placement of the medical device in the subject.
11. The method of claim 7 , wherein the α-sheet peptide comprises a peptide selected from the group consisting of:
AP90
SEQ ID NO: 1
RGEmNlSwMNEYSGWtMnLkMGR;
AP401
SEQ ID NO: 2
rGeMnLsWmneysGwTmNlKmGr;
AP407
SEQ ID NO: 3
RGEmNlCwMNEYSGWcMnLkMGR;
AP193
SEQ ID NO: 4
RGEmNyFwMNEYYGWtMnCkMGR;
and
AP5
SEQ ID NO: 8
RGNwNeSkMNEYSGWmLmLtMGR;
wherein residues in lower-case are D amino acids, residues in upper case are L amino acids and G residues are achiral.
12. A medical device comprising one or more a-sheet peptides coated on a surface of the medical device.
13. The medical device of claim 12 , the medical device is selected from the group consisting of prosthetic heart valves, cardiac pacemakers, cerebrospinal fluid shunts, urinary catheters, intravascular catheters, ocular prostheses, prosthetic joints, orthopedic implants, titanium-containing implants, polystyrene-containing implants, and intrauterine contraceptive devices.
14. The medical device of claim 12 , wherein the a-sheet peptide comprises a peptide selected from the group consisting of:
AP90
(SEQ ID NO: 1)
RGEmNlSwMNEYSGWtMnLkMGR;
AP401
(SEQ ID NO: 2)
rGeMnLsWmneysGwTmNlKmGr;
AP407
(SEQ ID NO: 3)
RGEmNlCwMNEYSGWcMnLkMGR;
AP193
(SEQ ID NO: 4)
RGEmNyFwMNEYYGWtMnCkMGR;
and
AP5
(SEQ ID NO: 8)
RGNwNeSkMNEYSGWmLmLtMGR;
wherein residues in lower-case are D amino acids, residues in upper case are L amino acids, and G residues are achiral.