Methods and compositions for modifying assembly-activating protein (AAP)-dependence of viruses
View Patent ↗Methods and compositions are provided that can be used to modify the assembly activating protein (AAP)-dependence of an adeno-associated virus (AAV).
1. An adeno-associated virus (AAV) capsid polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 3.
2. The AAV capsid polypeptide of claim 1 , having the amino acid sequence of SEQ ID NO:3.
3. The AAV capsid polypeptide of claim 1 , encoded by the nucleic acid sequence of SEQ ID NO: 4.
4. A virus particle comprising the adeno-associated virus (AAV) capsid polypeptide of claim 1 .
5. The virus particle of claim 4 , further comprising a transgene.
6. A nucleic acid molecule comprising a nucleic acid sequence encoding the adeno-associated virus (AAV) capsid polypeptide of claim 1 .
7. The nucleic acid molecule of claim 6 , having the nucleic acid sequence of SEQ ID NO: 4.
8. The nucleic acid molecule of claim 6 , encoding the amino acid sequence of SEQ ID NO: 3.
9. A vector comprising the nucleic acid molecule of claim 6 .
10. A host cell comprising the nucleic acid molecule of claim 6 .
11. The host cell of claim 10 , wherein the host cell is a packaging cell.
12. A packaging cell comprising a nucleic acid molecule encoding the adeno-associated virus (AAV) capsid polypeptide of claim 1 .
13. The packaging cell of claim 12 , wherein the packaging cell lacks an assembly activating protein (AAP).
14. An AAV capsid polypeptide comprising the amino acid sequence of SEQ ID NO:1 comprising amino acid substitutions K163T, A2065, K478R, L481V, V520M, T528S, L586N, A592Q, P593A, N599H, S600N, and A603V.