Microbiome-based informed method to formulate live biotherapeutics
Methods of formulating live biotherapeutics are disclosed in which a deficiency or excess of a specific bacterial strain in a person's microbiome is identified by comparing a gene-specific characterization of the person's microbiome against a comprehensive, non-redundant reference gene catalog, and the biotherapeutic is formulated by selecting bacteria to address the deficiency or excess. Embodiments include the formulation of live biotherapeutics for improving the health of a person's vaginal microbiome, i.e. using a vaginal reference gene catalog, and may be suitable for ameliorating, treating, or preventing a malignancy such as a cancer of the female genitourinary system.
1. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii , and
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express at least one of the asparagine synthase B (asnB) gene of SEQ ID NO: 1 and the asparagine synthase B (asnB) gene of SEQ ID NO: 2; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ ID NO: 3 and a polypeptide of SEQ ID NO: 4.
2. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject, wherein step (a) comprises at least one sub-step selected from the group consisting of:
(i) preprocessing one or more samples by performing at least one additional procedure selected from the group consisting of removing human contaminants from a sample, quality-filtering, and removing ribosomal RNA;
(ii) assembling at least one metagenome from each of one or more samples by executing a procedure to generate one or more de novo assemblies;
(iii) compiling coding DNA sequences (CDSs);
(iv) applying at least one taxonomic annotation by transitive assignment of species name from reads mapping to contigs; and
(v) applying at least one functional annotation using annotations from one or more functional databases; wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii , and
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express the asparagine synthase B (asnB) gene of SEQ ID NO: 1; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ ID NO: 3 and a polypeptide of SEQ ID NO: 4.
3. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii , and
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express the asparagine synthase B (asnB) gene of SEQ ID NO: 2; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ ID NO: 3 and a polypeptide of SEQ ID NO: 4;
wherein the live biotherapeutic formulation further comprises a pharmaceutically acceptable carrier.
4. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii , and
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express the asparagine synthase B (asnB) gene of SEQ ID NO: 1; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ ID NO: 3;
wherein the live biotherapeutic formulation further comprises an agent adapted to reduce or remove free ammonia from the vaginal environment.
5. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii;
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express at least one of the asparagine synthase B (asnB) gene of SEQ ID NO: 1 and the asparagine synthase B (asnB) gene of SEQ. ID NO: 2; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ. ID NO: 3 and a polypeptide of SEQ. ID NO: 4;
wherein step (a) comprises at least one sub-step selected from the group consisting of:
(i) preprocessing one or more samples by performing at least one additional procedure selected from the group consisting of removing human contaminants from a sample, quality-filtering, and removing ribosomal RNA;
(ii) assembling at least one metagenome from each of one or more samples by executing a procedure to generate one or more nucleotide based de novo assemblies;
(iii) compiling coding DNA sequences (CDSs);
(iv) applying at least one taxonomic annotation by transitive assignment of species name from reads mapping to contigs; and
(v) applying at least one functional annotation using annotations from one or more functional databases.
6. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii;
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express at least one of the asparagine synthase B (asnB) gene of SEQ ID NO: 1 and the asparagine synthase B (asnB) gene of SEQ. ID NO: 2; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ. ID NO: 3;
wherein the live biotherapeutic formulation further comprises a pharmaceutically acceptable carrier.
7. A method for treating bacterial vaginosis in a female human subject, comprising:
(a) generating a gene-specific characterization, at an intraspecies level, of the subject's vaginal microbial community;
(b) comparing the gene-specific characterization to a reference gene catalog of the human vaginal microbiome, wherein the reference gene catalog comprises at least one metagenome or single-strain genome associated with a healthy microbiome;
(c) identifying, based on the comparison of step (b), a deficiency or excess of at least one bacterial strain in the subject's vaginal microbial community;
(d) formulating a remedial live biotherapeutic formulation, comprising bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community; and
(e) administering the remedial live biotherapeutic formulation to the subject,
wherein the bacteria adapted to remedy the deficiency or excess of the at least one bacterial strain in the subject's vaginal microbial community comprise a selected strain or consortium of strains of a bacterial species selected from the group consisting of Lactobacillus crispatus, Lactobacillus gasseri , and Lactobacillus jensenii , and
wherein the bacteria comprise at least one of:
(i) Lactobacillus crispatus bacteria configured to express at least one of the asparagine synthase B (asnB) gene of SEQ ID NO: 1 and the asparagine synthase B (asnB) gene of SEQ. ID NO: 2; and
(ii) Lactobacillus crispatus bacteria containing an asparagine synthase B (asnB) gene encoding at least one of a polypeptide of SEQ. ID NO: 4;
wherein the live biotherapeutic formulation further comprises an agent adapted to reduce or remove free ammonia from the vaginal environment.