Smart pellet for sample testing
A submersible sensor device configured as a small pellet for testing biological and other liquid samples is provided. In one aspect, a sensing device includes: a housing; and one or more sensors contained within the housing, wherein the housing hermetically seals the sensors such that the sensing device is fully submersible in a liquid analyte. A method and system for analysis of a liquid sample using the present sensing device are also provided.
1. A sensing device, comprising: a housing; and sensors comprising two or more types of sensors contained within the housing, wherein the housing hermetically seals the sensors such that the sensing device and the sensors are fully submersible in a liquid analyte, wherein the sensing device once submerged in the liquid analyte is configured to collect data via the sensors and transmit the data, and wherein the sensors comprise at least one strain gauge comprising:
a sensor housing having a cavity therein; a strain sensor on a bottom of the cavity; a float positioned within the cavity in contact with the strain sensor; and
at least one optical sensor comprising: an immunoassay test strip.
2. The sensing device of claim 1 , wherein the housing comprises a flat top surface and a flat bottom surface opposite the flat top surface, and wherein the flat top surface and the flat bottom surface are both circular such that the sensing device is shaped as a pellet.
3. The sensing device of claim 2 , wherein the pellet has a diameter d of less than or equal to about 10 cm.
4. The sensing device of claim 3 , wherein the diameter d is less than or equal to about 2 cm.
5. The sensing device of claim 4 , wherein the diameter d is from about 0.5 cm to about 1 cm and ranges therebetween.
6. The sensing device of claim 2 , wherein the pellet has a thickness t of from about 1.25 mm to about 25 mm and ranges therebetween.
7. The sensing device of claim 1 , wherein at least a portion of the housing is transparent.
8. The sensing device of claim 1 , wherein the two or more types of sensors comprise sensors selected from the group consisting of: electrical sensors, electrochemical sensors, optical sensors, pressure sensors, temperature sensors, specific gravity sensors, acoustic sensors, and combinations thereof.
9. The sensing device of claim 1 , wherein the at least one optical sensor comprises the immunoassay test strip on a backing substrate; a light source; a light detector, wherein the light source and light detector are oriented orthogonal to the immunoassay test strip: and a prism between the immunoassay test strip and the light source and light detector.
10. The sensing device of claim 9 , wherein the light source comprises a light emitting diode and the light detector comprises a photodiode.
11. The sensing device of claim 9 , wherein the immunoassay test strip comprises multiple test pads, and wherein the at least one optical sensor further comprises:
a separator between each of the test pads, wherein the separator comprises a polymer that swells when wet.
12. The sensing device of claim 9 , wherein the at least one optical sensor further comprises:
a layer of a superabsorbent polymer between the immunoassay test strip and the backing substrate.
13. The sensing device of claim 1 , wherein the at least one optical sensor comprises: a microfluidic channel coated with a reagent-specific marker: an inlet at a first end of the microfluidic channel; an imaging device at a second end of the microfluidic channel opposite the inlet; a light source adjacent to the first end of the microfluidic channel; and a waveguide for directing light from the light source to the imaging device.
14. The sensing device of claim 13 , wherein the imaging device comprises a camera on a chip.
15. The sensing device of claim 1 , wherein a top of the float is recessed below a top surface of the sensor housing.
16. The sensing device of claim 1 , wherein the strain gauge further comprises:
a vent line leading from the bottom of the cavity out to an opposite side of the sensor housing from the cavity.
17. The sensing device of claim 1 , wherein the float comprises at least one protrusion that compliments a track along a wall of the cavity which prevents any rotation of the float while permitting the float to move freely up and down within the cavity.
18. A sensing device, comprising: a housing; and sensors comprising two or more types of sensors hermetically sealed within the housing such that the sensing device and the sensors are fully submersible in a liquid analyte, wherein the housing comprises a flat top surface and a flat bottom surface opposite the flat top surface, wherein the flat top surface and the flat bottom surface are both circular such that the sensing device is shaped as a pellet having a diameter d of less than or equal to about 10 cm, and a thickness t of from about 1.25 mm to about 25 mm and ranges therebetween, wherein the sensing device once submerged in the liquid analyte is configured to collect data via the sensors and transmit the data, and wherein the sensors comprise at least one strain gauge comprising: a sensor housing having a cavity therein; a strain sensor on a bottom of the cavity; a float positioned within the cavity in contact with the strain sensor; and at least one optical sensor comprising: an immunoassay test strip.
19. A system for analysis of a liquid sample, the system comprising: at least one sensing device comprising a housing, and sensors comprising two or more types of sensors contained within the housing, wherein the housing hermetically seals the sensors such that the sensing device and the sensors are fully submersible in the liquid sample, and wherein the sensors comprise at least one strain gauge comprising: a sensor housing having a cavity therein; a strain sensor on a bottom of the cavity; a float positioned within the cavity in contact with the strain sensor; and at least one optical sensor comprising: an immunoassay test strip; and a data management system communicatively connected to the at least one sensing device, wherein the at least one sensing device once submerged in the liquid sample is configured to collect data via the sensors and transmit the data to the data management system.
20. The system of claim 19 , wherein the two or more types of sensors comprise sensors selected from the group consisting of: electrical sensors, electrochemical sensors, optical sensors, pressure sensors, temperature sensors, specific gravity sensors, acoustic sensors, and combinations thereof.
21. The sensing device of claim 1 , wherein the sensing device once submerged in the liquid analyte is configured to wirelessly transmit the data to a data management system.
22. The sensing device of claim 1 , wherein the sensing device is configured to collect a unitized amount of a sample from the liquid analyte to a sensing surface comprising the sensors.