Bioadhesive for corneal repair
The present invention provides compositions and methods for repair and reconstruction of defects and injuries to the cornea.
1. A composition for corneal or scleral reconstruction or repair comprising a methacryloyl-substituted gelatin, a visible light activated photoinitiator, and a pharmaceutically acceptable carrier, wherein the methacryloyl-substituted gelatin comprises methacrylamide substitution and methacrylate substitution, and a ratio of methacrylamide substitution to methacrylate substitution in the methacryloyl-substituted gelatin is between 80:20 and 99:1.
2. The composition of claim 1 , wherein the methacryloyl-substituted gelatin has a degree of methacryloyl substitution between 30% and 85%.
3. The composition of claim 1 , wherein the methacryloyl-substituted gelatin is present at a concentration between 5% and 25% (w/v).
4. The composition of claim 3 , wherein the methacryloyl-substituted gelatin is present at a concentration between 8% and 12% (w/v) or between 17% and 23% (w/v).
5. The composition of claim 4 , wherein the methacryloyl-substituted gelatin is present at a concentration of about 10% (w/v) or of about 20% (w/v).
6. The composition of claim 1 , wherein the visible light activated photoinitiator is selected from the group consisting of: Eosin Y, triethanolamine, vinyl caprolactam, dl-2,3-diketo-1,7,7-trimethylnorcamphane (CQ), 1-phenyl-1,2-propadione (PPD), 2,4,6-trimethylbenzoyl-diphenylphosphine oxide (TPO), bis(2,6-dichlorobenzoyl)-(4-propylphenyl)phosphine oxide (Ir819), 4,4′-bis(dimethylamino)benzophenone, 4,4′-bis(diethylamino)benzophenone, 2-chlorothioxanthen-9-one, 4-(dimethylamino)benzophenone, phenanthrenequinone, ferrocene, diphenyl(2,4,6 trimethylbenzoyl)phosphine oxide/2-hydroxy-2-methylpropiophenone (50/50 blend), dibenzosuberenone, (benzene) tricarbonylchromium, resazurin, resorufin, benzoyltrimethylgermane, and any combination thereof.
7. The composition of claim 1 , wherein the visible light activated photoinitiator is a mixture comprising Eosin Y, triethanolamine, and vinyl caprolactam.
8. The composition of claim 7 , wherein a concentration of Eosin Y is between 0.0125 and 0.5 mM, a concentration of triethanolamine is between 0.1 and 2% w/v, and a concentration of vinyl caprolactam is between 0.05 and 1.5% w/v.
9. The composition of claim 8 , wherein: (i) the concentration of Eosin Y is about 0.1 mM, the concentration of triethanolamine is about 0.5% w/v, and the concentration of vinyl caprolactam is about 0.5% w/v; or (ii) the concentration of Eosin Y is about 0.05 mM, the concentration of triethanolamine is about 0.4% w/v, and the concentration of vinyl caprolactam is about 0.4% w/v.
10. The composition of claim 1 , wherein the composition further comprises corneal cells.
11. The composition of claim 1 , wherein the composition further comprises a therapeutic agent.
12. A method for corneal or scleral reconstruction or repair, comprising the steps of:
a. applying a composition of claim 1 to a corneal or scleral defect; and
b. exposing the composition to visible light.