Self-assembly of protein-based biomaterials with multiple morphologies
The present disclosure relates, in some embodiments, to a composition comprising a biomaterial. A biomaterial may comprise, for example, one or more molecules capable of self-association and/or self-assembly. In some embodiments, a biomaterial may comprise one or more polypeptides and/or proteins. A biomaterial may comprise, for example, two or more self-assembled Ultrabithorax (Ubx) protein molecules. A Ubx protein, in some embodiments, may be any wild type Drosophila melanogaster Ultrabithorax protein, including any natural or non-natural isoforms (e.g., alternative splicing isoforms). The present disclosure relates, in some embodiments, to a method of making a biomaterial comprising contacting two or more Ubx protein molecules under conditions that permit self-assembly to form a first fibril and contacting the first fibril to a second fibril.
1. A biomaterial composition comprising two or more self-assembled Ultrabithorax (Ubx) proteins, wherein at least one of the two or more self-assembled Ubx proteins is a fusion protein in which the Ubx protein is fused with at least one other protein.
2. The biomaterial composition of claim 1 , wherein the two or more self-assembled Ubx proteins comprise amino acid sequences selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8.
3. The biomaterial composition of claim 1 , wherein the Ubx protein further comprises a ligand.
4. The biomaterial composition of claim 3 , wherein the ligand is selected from the group consisting of labeled markers, growth factors, transcription factors, antibodies, cytokines, hormones, antibiotics, and combinations thereof.
5. The biomaterial composition of claim 3 , wherein the ligand is biotin.
6. The biomaterial composition of claim 1 , wherein the Ubx protein is a fusion protein comprising at least two other proteins.
7. The biomaterial composition of claim 1 wherein the other protein is selected from the group consisting of all or part of an enzyme, an enzyme inhibitor, an antigen, an antibody, a hormone, a coagulation factor, an interferon, a cytokine, a growth factor, differentiation factor, an osteogenic factor, a bone resorption factor, a cellular motility factor, a bactericidal factor, an antifungal factor, a chemotactic factor, a cytostatic factor, a plasma adhesive molecule, an interstitial adhesive molecule, an extracellular matrix, and a ligand binding factor.
8. The biomaterial composition of claim 1 wherein the Ubx protein is selected from the group consisting of SEQ ID NO: 3, a deletion mutant of SEQ ID NO: 3 missing residues 19-48, residues 216-356 of SEQ ID NO: 3, and residues 88-243 of SEQ ID NO: 3.
9. The biomaterial composition of claim 1 wherein the Ubx protein is at least 95% identical to full length SEQ ID NO: 3.
10. A biomaterial structure having a fibril, film, fiber, sheet, bundle, lattice, scaffold or encapsulate morphology and comprising the biomaterial composition of claim 1 .
11. The biomaterial composition of claim 10 wherein the structure comprises at least two different Ubx fusion proteins.
12. The biomaterial composition of claim 11 wherein the structure is a bundle that comprises at least two different Ubx fusion protein fibers.