IP Library › Granted Patent US 11,060,060
Granted Patent B2
US 11,060,060 · App. 15/760,452 · Granted Jul 13, 2021

Method of making trabecular bone

Inventors: Harry Colbert Blair (Pittsburgh, PA); Quitterie Larrouture (Pittsburgh, PA); Irina L. Tourkova (Pittsburgh, PA); Deborah J. Nelson (Riverside, IL); Paul Schlesinger (University City, MO)
Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education; The United States Government as represented by the Department of Veterans Affairs; University of Chicago; Washington University
C12N5/0654A01K67/0276C12N15/1138A01K2217/058A01K2217/075C12N2310/14C12N2310/531C12N2501/999C12N2506/1346C12N2510/00C12N2517/00C12N2517/02
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Quick Facts
Patent No.
US 11,060,060
App. No.
15/760,452
Granted
Jul 13, 2021
Kind
B2
Abstract

Provided herein are methods of producing trabecular bone material, such as trabecular bone or trabecular bone matrix and cells useful in preparing the trabecular bone material.

Claims (10)

1. A method of producing trabecular bone material, comprising: culturing CLC3 knockout osteoblasts ex vivo in culture medium for a length of time such that trabecular bone material is produced.

2. The method of claim 1 , wherein the CLC3 knockout osteoblasts are obtained by culturing CLC3 knockout mesenchymal stem cells or osteoblast precursor cells in osteoblast differentiation medium.

3. The method of claim 2 , wherein the mesenchymal stem cells or osteoblast precursor cells are human or murine.

4. The method of claim 2 , wherein the osteoblast differentiation medium comprises ascorbic acid and a phosphate source.

5. The method of claim 4 , wherein the osteoblast differentiation medium does not contain a glucocorticoid.

6. The method of claim 4 , wherein the phosphate source is glycerol-2-phosphate.

7. The method of claim 1 , wherein the osteoblasts are human or murine.

8. The method of claim 1 , wherein the CLC3 knockout knockdown osteoblasts are prepared by:

a. isolating CLC3 knockout mesenchymal stem cells or osteoblast precursor cells from a CLC 3 knockout transgenic animal; and

b. differentiating the CLC3 knockout mesenchymal stem cells or osteoblast precursor cells to an osteoblast phenotype, thereby producing a CLC3 knockout osteoblast capable of producing trabecular bone material.

Assignments (6)
CONFIRMATORY LICENSE Recorded Sep 13, 2021
From: UNIVERSITY OF PITTSBURGH
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 057494/0436 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 8, 2021
From: LARROUTURE, QUITTERIE
To: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
Reel/Frame 056471/0447 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 8, 2021
From: TOURKOVA, IRINA L.
To: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION; THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
Reel/Frame 056471/0518 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 8, 2021
From: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
To: TOURKOVA, IRINA L.
Reel/Frame 056471/0824 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 8, 2021
From: BLAIR, HARRY COLBERT
To: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION; THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
Reel/Frame 056472/0034 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 5, 2019
From: SCHLESINGER, PAUL
To: WASHINGTON UNIVERSITY
Reel/Frame 049674/0555 →
Continuity (2)
Provisional Application 62219480 · Sep 16, 2015
Related Publication 20180258399A1 · Sep 13, 2018