Automated set-up for cell sorting
Apparatus and methods are described for automatically performing set-up steps for flow cytometry operations. The invention provides for the spatial determination of a flow stream and the subsequent automatic alignment of analysis devices and/or collection vessels. The automatic determination of flow stream properties provides for the automatic configuration flow cytometer parameters.
1. A method for adjusting one or more parameters of a flow cytometer comprising a flow cell, the method comprising:
detecting light from a flow stream in the flow cytometer;
determining the width of the flow stream in a detection field;
generating a data signal corresponding to a ratio of the determined width of the flow stream and a width of the flow stream that is expected based on the flow cell nozzle orifice site; and
automatically adjusting one or more parameters of the flow cytometer in response to the generated data signal,
wherein the light from the flow stream is detected at a droplet break-off point of the flow stream, and
wherein the width of the flow stream is determined based on droplet volume.
2. The method according to claim 1 , wherein the one or more parameters of the flow cytometer is selected from the group consisting of hydrostatic pressure, sheath fluid pressure, flow stream charge, deflection voltage, drop drive frequency, charge correction value, drop delay, drop amplitude and charge phase.
3. The method according to claim 1 , wherein the processor is configured to adjust sheath fluid pressure based on the determined flow cell nozzle orifice size.
4. The method according to claim 1 , wherein the processor is configured to adjust drop drive frequency based on the determined flow cell nozzle orifice size.
5. The method according to claim 1 , wherein the method comprises determining the nozzle orifice size of the flow cell.
6. The method according to claim 5 , wherein the method further comprises generating a data signal corresponding to an expected flow cell nozzle orifice size based on the determined width of the flow stream and adjusting one or more parameters of the flow cytometer based on the determined flow cell nozzle orifice size.
7. The method according to claim 1 , wherein light from the flow stream is detected with a charge-coupled device (CCD) camera.
8. A flow cytometer comprising:
a flow cell comprising a nozzle orifice that propagates a sample in a flow stream;
a detector that detects light from the flow stream;
a processor comprising memory operably coupled to the processor, wherein the memory comprises instructions stored thereon, the instructions are executed by the processor to:
determine the width of the flow stream in a detection field;
generate a data signal corresponding to a ratio of the determined width of the flow stream and a width of the flow stream that is expected based on the size of the flow cell nozzle orifice; and
automatically adjust one or more parameters of the flow cytometer in response to the generated data signal,
wherein the light from the flow stream is detected at a droplet break-off point of the flow stream, and
wherein the width of the flow stream is determined based on droplet volume.
9. The flow cytometer according to claim 8 , wherein the processor is configured to automatically adjust sheath fluid pressure based on the determined flow cell nozzle orifice size.
10. The flow cytometer according to claim 9 , wherein the processor is configured to automatically adjust drop drive frequency based on the determined flow cell nozzle orifice size.
11. The flow cytometer according to claim 8 , wherein the parameters of the flow cytometer is selected from the group consisting of hydrostatic pressure, sheath fluid pressure, flow stream charge, deflection voltage, drop drive frequency, charge correction value, drop delay, drop amplitude and charge phase.
12. The flow cytometer according to claim 8 , wherein the processor is configured to determine the nozzle orifice diameter of the flow cell.
13. The flow cytometer according to claim 12 , wherein the processor is configured to generate a data signal corresponding to an expected flow cell nozzle orifice size based on the determined width of the flow stream.
14. The flow cytometer according to claim 13 , wherein the processor is configured to automatically adjust one or more parameters of the flow cytometer based on the determined flow cell nozzle orifice size.
15. The flow cytometer according to claim 8 , wherein the detector that detects light from the flow stream is a charge-coupled device (CCD) camera.