Recombinant gram negative bacteria and methods of generating and utilizing same
The present invention provides novel, recombinant Gram-negative bacteria. In particular, the invention provides recombinant Gram-negative bacteria (e.g., E. coli ) lacking genes involved in lipopolysaccharide (LPS, endotoxin) biosynthesis (e.g., lacking genes required for core oligosaccharide biosynthesis) and also provides recombinant Gram-negative bacteria lacking genes involved in LPS biosynthesis that contain one or more exogenous KDO transferases and/or one or more exogenous heptosyltransferases (e.g., from one or more types and/or strains of bacteria). The invention further provides methods of generating and utilizing (e.g., as or in an immunogenic composition (e.g., as or in an adjuvant and/or vaccine)) the recombinant Gram-negative bacteria therapeutic, preventative, and/or research applications.
1. A recombinant E. coli cell lacking all endogenous glycosyltransferases involved in the biosynthesis of the core oligosaccharide within lipopolysaccharide (LPS), wherein the cell comprises an exogenous KDO transferase.
2. The recombinant E. coli cell of claim 1 , wherein the cell lacks endogenous waaF, waaC, waaL, waaU, waaZ, waaY, waaJ, waaR, waaB, waaS, waaP, waaG, waaQ, and waaA genes.
3. The recombinant E. coli cell of claim 1 , wherein the cell possesses endogenous hldD and coaD genes.
4. The recombinant E. coli cell of claim 1 , wherein the cell generates sugar precursors of core oligonucleotide biosynthesis but lacks the capability to transfer the sugar precursors to the Lipid IV A molecule.
5. The recombinant E. coli cell of claim 1 , wherein the exogenous KDO transferase is from H. influenzae.
6. The recombinant E. coli cell of claim 1 , wherein the KDO transferase is from C. trachomatis.
7. The recombinant E. coli cell of claim 1 , wherein the KDO transferase is from Chlamydophila psittaci.
8. The recombinant E. coli cell of claim 1 , further comprising an exogenous heptosyltransferase.
9. The recombinant E. coli cell of claim 8 , wherein the heptosyltransferase is from Helicobacter pylori.
10. The recombinant E. coli cell of claim 8 , wherein the heptosyltransferase is from Campylobacter jejuni.
11. The recombinant E. coli cell of claim 8 , wherein the heptosyltransferase is from Aquifex aeolicus.
12. A recombinant E. coli cell lacking all endogenous glycosyltransferases involved in the biosynthesis of the core oligosaccharide within lipopolysaccharide (LPS), wherein the cell comprises one or more exogenous KDO transferases.
13. The recombinant E. coli cell of claim 12 , further comprising one or more exogenous heptosyltransferases.