Light detection systems and methods for using thereof
Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include two or more photodetector arrays and an optical adjustment component positioned in an optical path between the photodetector arrays. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having two or more photodetector arrays and an optical adjustment component are also provided.
1. A method comprising:
detecting light from the flow stream with a light detection system comprising:
a first photodetector array in optical communication with a second photodetector array, wherein the first photodetector array and second photodetector array each comprises two or more photodetectors and an array optical adjustment component configured to propagate light between the photodetectors in the array; and
an optical adjustment component positioned in an optical path between the first photodetector array and the second photodetector array.
2. The method according to claim 1 , further comprising irradiating a sample in a flow stream in an interrogation field with a light source.
3. The method according to claim 1 , wherein the flow stream is irradiated with a light source at a wavelength from 200 nm to 800 nm.
4. The method according to claim 2 , wherein the light source is a laser.
5. The method according to claim 2 , wherein light from the flow stream is propagated to the light detection system with an optical collection component.
6. The method according to claim 5 , wherein the optical collection component comprises fiber optics.
7. The method according to claim 6 , wherein the optical collection component comprises a fiber optics light relay bundle.
8. The method according to claim 5 , wherein the optical collection component comprises a free-space light relay system.
9. The method according to claim 1 , further comprising measuring the detected light at one or more wavelengths.
10. A kit comprising:
two or more photodetector arrays, each photodetector array comprising two or more photodetectors and an array optical adjustment component configured to propagate light between the photodetectors in the array; and
an optical adjustment component configured for positioning in an optical path between each photodetector array.
11. The kit according to claim 10 , wherein the array optical adjustment component comprises a collimator.
12. The kit according to claim 10 , wherein the array optical adjustment component comprises a beam splitter.
13. The kit according to claim 10 , wherein the array optical adjustment component comprises a dichroic mirror.
14. The kit according to claim 10 , wherein the photodetectors are photomultiplier tubes.
15. The kit according to claim 10 , wherein the photomultiplier tube is a metal package photomultiplier tube.
16. The method according to claim 1 , wherein the first and second photodetector arrays are arranged in a linear configuration.
17. The method according to claim 1 , wherein the first and second photodetector arrays are arranged in a concentric configuration.
18. The method according to claim 1 , wherein the array optical adjustment component is selected from the group consisting of a lens, mirror, beam splitter, collimating lens, pinhole, slit, grating and light refractor.
19. The method according to claim 18 , wherein the array optical adjustment component is a mirror.