CD80 extracellular domain polypeptides and their use in cancer treatment
This application relates to CD80 (B7-1) extracellular domain (ECD) polypeptides and CD80-ECD fusion molecules and their use in treatment of cancer, both alone and in combination with other therapeutic agents, such as immune stimulating agents such as PD-1/PD-L1 inhibitors.
1. An isolated polynucleotide comprising a nucleic acid sequence that encodes a CD80 extracellular domain (ECD) fusion molecule comprising the polypeptide sequence of SEQ ID NO:20.
2. The isolated polynucleotide of claim 1 , wherein the nucleic acid sequence further encodes a leader sequence.
3. A vector comprising the polynucleotide of claim 1 .
4. A vector comprising the polynucleotide of claim 2 .
5. A host cell comprising the vector of claim 3 .
6. A host cell comprising the vector of claim 4 .
7. A method of obtaining a CD80 ECD fusion protein comprising isolating the fusion molecule expressed by the host cell of claim 5 .
8. A method of obtaining a CD80 ECD fusion protein comprising isolating the fusion molecule expressed by the host cell of claim 6 .