Kit and method for detecting HSV1 and HSV2
View Patent ↗A kit for simultaneously detecting HSV1 and HSV2 includes a forward primer and a reverse primer specific to HSV1, and a forward primer and a reverse primer specific to HSV2. The forward primer specific to HSV1 has a sequence of SEQ ID NO: 1 or SEQ ID NO: 4, and the reverse primer specific to HSV1 has a sequence of SEQ ID NO: 2 or SEQ ID NO: 7. The forward primer specific to HSV2 has a sequence of SEQ ID NO: 6, and the reverse primer specific to HSV2 has a sequence of SEQ ID NO: 7.
1. A method for simultaneously or independently detecting HSV1 and/or HSV2, the method comprising the step of amplifying DNA from HSV1 and/or HSV2 using real-time polymerase chain reaction with at least one primer pair specific to HSV1 and/or HSV2,
wherein the primer pair specific to HSV1 consists of a forward primer of SEQ ID NO: 1 and a reverse primer of SEQ ID NO: 2, or consists of a forward primer of SEQ ID NO: 4 and a reverse primer of SEQ ID NO: 7; and
wherein the primer pair specific to HSV2 consists of a forward primer of SEQ ID NO: 6 and a reverse primer of SEQ ID NO: 7.
2. The method according to claim 1 wherein a probe specific to HSV1 is used in the real-time polymerase chain reaction and has a sequence of SEQ ID NO: 3, or a sequence complementary to SEQ ID NO: 3.
3. The method according to claim 1 wherein a probe specific to HSV1 is used in the real-time polymerase chain reaction and has a sequence of SEQ ID NO: 5, or a sequence complementary to SEQ ID NO: 5.
4. The method according to claim 1 wherein a probe specific to HSV2 is used in the real-time polymerase chain reaction and has a sequence of SEQ ID NO: 8 or a sequence complementary to SEQ ID NO: 8.