Polypeptides binding to human complement C5

1. A C5 binding polypeptide comprising:

(i) a C5 binding motif, BM, which motif: (a) forms part of a three-helix bundle protein domain and (b) comprises an amino acid sequence:

EX 2 X 3 X 4 A X 6 X 7 EID X 11 LPNL X 16 X 17 X 18 QW X 21 AFIX 25 X 26 LX 28 D (SEQ ID NO. 763);

wherein, independently of each other,

X 2 is selected from H, Q, S, T and V;

X 3 is selected from I, L, M and V;

X 4 is selected from A, D, E, H, K, L, N, Q, R, S, T and Y;

X 6 is selected from N and W;

X 7 is selected from A, D, E, H, N, Q, R, S and T;

X 11 is selected from A, E, G, H, K, L, Q, R, S, T and Y;

X 16 is selected from N and T;

X 17 is selected from I, L and V;

X 18 is selected from A, D, E, H, K, N, Q, R, S and T;

X 21 is selected from I, L and V;

X 25 is selected from D, E, G, H, N, S and T;

X 26 is selected from K and S; and

X 28 is selected from A, D, E, H, N, Q, S, T and Y, and

iii) additional C terminal and/or N terminal amino acids to facilitate production, purification, stabilization in vivo or in vitro, coupling, or detection of the polypeptide.

2. The C5 binding polypeptide according to claim 1 , wherein the additional amino acids are selected from a cysteine residue, a His 6 tag, a myc tag, a FLAG tag, and a half-life extending moiety.

3. The C5 binding polypeptide according to claim 2 , wherein the additional amino acids comprise a half-life extending moiety and wherein the half-life extending moiety is an Fc domain of an antibody.

4. The C5 binding polypeptide according to claim 2 , wherein the additional amino acids comprise a half-life extending moiety and the half-life extending moiety is an albumin binding domain.

5. The C5 binding polypeptide according to claim 4 , wherein the albumin binding domain is an albumin binding domain of streptococcal protein G.

6. The C5 binding polypeptide according to claim 4 , wherein the albumin-binding domain comprises the amino acid sequence of SEQ ID NO:759.

7. The C5 binding polypeptide according to claim 1 , wherein the amino acid sequence fulfills at least four of the following eight conditions I-VIII:

I. X2 is V;

II. X3 is selected from I and L;

III. X6 is W;

IV. X7 is selected from D and N;

V. X17 is selected from I and L;

VI. X21 is L;

VII. X25 is N;

VIII. X28 is D.

8. The C5 binding polypeptide according to claim 1 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-248.

9. The C5 binding polypeptide according to claim 1 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12, SEQ ID NO:20, SEQ ID NOs:23-24, SEQ ID NOs:26-28, SEQ ID NOs:32-35, SEQ ID NOs:38-39, SEQ ID NO:41, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NOs:56-57, SEQ ID NO:59, SEQ ID NO:66, SEQ ID NOs:78-79, SEQ ID NO:87, SEQ ID NO:92, SEQ ID NO:106, SEQ ID NO:110, SEQ ID NO:119, SEQ ID NO:125, SEQ ID NO:141, SEQ ID NO:151, SEQ ID NO:161, SEQ ID NO:166, SEQ ID NO:187, SEQ ID NO:197, SEQ ID NO:203, SEQ ID NO:205, SEQ ID NO:215 and SEQ ID NO:243.

10. The C5 binding polypeptide according to claim 1 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12.

11. The C5 binding polypeptide according to claim 1 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-5.

12. The C5 binding polypeptide according to claim 2 , wherein the amino acid sequence fulfills at least four of the following eight conditions I-VIII:

I. X2 is V;

II. X3 is selected from I and L;

III. X6 is W;

IV. X7 is selected from D and N;

V. X17 is selected from I and L;

VI. X21 is L;

VII. X25 is N;

VIII. X28 is D.

13. The C5 binding polypeptide according to claim 2 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-248.

14. The C5 binding polypeptide according to claim 2 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12, SEQ ID NO:20, SEQ ID NOs:23-24, SEQ ID NOs:26-28, SEQ ID NOs:32-35, SEQ ID NOs:38-39, SEQ ID NO:41, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NOs:56-57, SEQ ID NO:59, SEQ ID NO:66, SEQ ID NOs:78-79, SEQ ID NO:87, SEQ ID NO:92, SEQ ID NO:106, SEQ ID NO:110, SEQ ID NO:119, SEQ ID NO:125, SEQ ID NO:141, SEQ ID NO:151, SEQ ID NO:161, SEQ ID NO:166, SEQ ID NO:187, SEQ ID NO:197, SEQ ID NO:203, SEQ ID NO:205, SEQ ID NO:215 and SEQ ID NO:243.

15. The C5 binding polypeptide according to claim 2 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12.

16. The C5 binding polypeptide according to claim 2 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-5.

17. The C5 binding polypeptide according to claim 3 , wherein the amino acid sequence fulfills at least four of the following eight conditions I-VIII:

I. X2 is V;

II. X3 is selected from I and L;

III. X6 is W;

IV. X7 is selected from D and N;

V. X17 is selected from I and L;

VI. X21 is L;

VII. X25 is N;

VIII. X28 is D.

18. The C5 binding polypeptide according to claim 3 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-248.

19. The C5 binding polypeptide according to claim 3 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12, SEQ ID NO:20, SEQ ID NOs:23-24, SEQ ID NOs:26-28, SEQ ID NOs:32-35, SEQ ID NOs:38-39, SEQ ID NO:41, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NOs:56-57, SEQ ID NO:59, SEQ ID NO:66, SEQ ID NOs:78-79, SEQ ID NO:87, SEQ ID NO:92, SEQ ID NO:106, SEQ ID NO:110, SEQ ID NO:119, SEQ ID NO:125, SEQ ID NO:141, SEQ ID NO:151, SEQ ID NO:161, SEQ ID NO:166, SEQ ID NO:187, SEQ ID NO:197, SEQ ID NO:203, SEQ ID NO:205, SEQ ID NO:215 and SEQ ID NO:243.

20. The C5 binding polypeptide according to claim 3 , wherein the amino acid sequence is selected from any one of SEQ ID NOs: 1-12.

21. The C5 binding polypeptide according to claim 3 , wherein the amino acid sequence is selected from any one of SEQ ID NOs: 1-5.

22. The C5 binding polypeptide according to claim 4 , wherein the amino acid sequence fulfills at least four of the following eight conditions I-VIII:

I. X2 is V;

II. X3 is selected from I and L;

III. X6 is W;

IV. X7 is selected from D and N;

V. X17 is selected from I and L;

VI. X21 is L;

VII. X25 is N;

VIII. X28 is D.

23. The C5 binding polypeptide according to claim 4 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-248.

24. The C5 binding polypeptide according to claim 4 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12, SEQ ID NO:20, SEQ ID NOs:23-24, SEQ ID NOs:26-28, SEQ ID NOs:32-35, SEQ ID NOs:38-39, SEQ ID NO:41, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NOs:56-57, SEQ ID NO:59, SEQ ID NO:66, SEQ ID NOs:78-79, SEQ ID NO:87, SEQ ID NO:92, SEQ ID NO:106, SEQ ID NO:110, SEQ ID NO:119, SEQ ID NO:125, SEQ ID NO:141, SEQ ID NO:151, SEQ ID NO:161, SEQ ID NO:166, SEQ ID NO:187, SEQ ID NO:197, SEQ ID NO:203, SEQ ID NO:205, SEQ ID NO:215 and SEQ ID NO:243.

25. The C5 binding polypeptide according to claim 4 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-12.

26. The C5 binding polypeptide according to claim 4 , wherein the amino acid sequence is selected from any one of SEQ ID NOs:1-5.

27. A polynucleotide encoding a C5 binding polypeptide according to claim 1 .

28. A polynucleotide encoding a C5 binding polypeptide according to claim 7 .

29. A polynucleotide encoding a C5 binding polypeptide according to claim 8 .

30. A polynucleotide encoding a C5 binding polypeptide according to claim 9 .

31. A polynucleotide encoding a C5 binding polypeptide according to claim 10 .

32. A polynucleotide encoding a C5 binding polypeptide according to claim 11 .

33. An expression vector comprising a polynucleotide encoding a C5 binding polypeptide according to claim 1 .

34. An expression vector comprising a polynucleotide encoding a C5 binding polypeptide according to claim 7 .

35. An expression vector comprising a polynucleotide encoding a C5 binding polypeptide according to claim 8 .

36. An expression vector comprising a polynucleotide encoding a C5 binding polypeptide according to claim 9 .

37. An expression vector comprising a polynucleotide encoding a C5 binding polypeptide according to claim 10 .