Device, system and kit for measuring tension of sheet-like tissue containing cardiomyocytes
A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.
1. A device for measuring a tension of tissue containing cardiomyocytes comprising: a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member so as to fix one end of a film of gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member, wherein the second gel adapter holder is attached to the first gel adapter holder so that the second gel holding member is opposed to the first gel holding member inside the frame member, and the second gel adapter holder has a mechanism for sliding in an axial direction in which the first gel holding member and the second gel holding member are opposed to each other; wherein the second gel adapter holder is detachably attached to the first gel adapter holder so that the second gel holding member is opposed to the first gel holding member inside the frame member, and wherein the connection member is provided with one or more L-shaped members for detachably attaching the second gel adapter holder to the first gel adapter holder wherein the L-shaped member is fitted in a first guide groove provided in the frame member.
2. The device according to claim 1 , wherein the first gel holding member has one or more first gel holding openings and the second gel holding member has one or more second gel holding openings.
3. The device according to claim 2 , wherein two to five first gel holding openings and two to five second gel holding openings are provided.
4. The device according to claim 1 , wherein the thicknesses of the first gel holding member and the second gel holding member are thinner than the thickness of the frame member.
5. The device according to claim 1 , wherein the thickness of the frame member is from 0.5 mm to 3.0 mm.
6. The device according to claim 1 , wherein the first gel adapter holder is provided with a stopper for restricting the slidable range of the second gel adapter holder.
7. The device according to claim 1 , wherein the film of gel is provided between the first gel holding member and the second gel holding member.
8. The device according to claim 7 , wherein the gel is a hydrogel.
9. The device according to claim 7 , wherein the gel is a fibrin gel.
10. The device according to claim 7 , wherein the tissue containing cardiomyocytes is adhered to the film of gel.
11. The device according to claim 10 , wherein the tissue is a cell sheet.
12. A system for measuring a tension of tissue containing cardiomyocytes comprising:
the device according to claim 10 ;
a culture medium tank into which the device is to be dipped;
a tension detection unit which is connected to the connection member of the second gel adapter holder;
an arithmetic unit which is connected to the tension detection unit, arithmetically processes a signal detected by the tension detection unit, and calculates the tension; and
an output unit which displays the result calculated by the arithmetic unit.
13. The system according to claim 12 , wherein the tension detection unit is a load cell.