Methods and compositions for
The present disclosure provides for unique spinach plants with broad-spectrum resistance to downy mildew and their progeny. Such plants may comprise an introgressed QTL associated with the broad-spectrum resistance to downy mildew. In certain aspects, compositions, including distinct polymorphic molecular markers, and methods for producing, using, identifying, selecting, and the like of plants or germplasm with resistance to downy mildew are provided.
1. A recombined chromosomal segment comprising an allele from Spinacia tetrandra conferring broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae (Pfs) and at least a first genetic locus from Spinacia oleracea , wherein said allele confers broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae when present in a spinach plant, wherein the broad-spectrum resistance comprises resistance to races 7, 10, 11, 12, 13, and 14 of Peronospora farinosa f. sp. spinaciae (Pfs), wherein said allele comprises sequences at least 99% identical to SEQ ID NO:1 and SEQ ID NO:2, wherein a representative sample of seed comprising said allele has been deposited under Accession No. PTA-120533 or Accession No. PTA-120534.
2. The chromosomal segment of claim 1 , further defined as lacking all or some genetic loci that are genetically linked to said allele in Spinacia tetrandra.
3. A Spinacia oleracea spinach plant comprising the chromosomal segment of claim 1 .
4. A Spinacia oleracea spinach seed comprising the chromosomal segment of claim 1 .
5. A Spinacia oleracea spinach cell comprising the chromosomal segment of claim claim 1 .