Non-naturally occurring porcine reproductive and respiratory syndrome virus (PRRSV) and methods of using
A non-naturally occurring porcine reproductive and respiratory syndrome virus (PRRSV) is provided herein, and methods of making and using the non-naturally occurring PRRSV also are provided.
1. A porcine reproductive and respiratory syndrome virus (PRRSV)-CON polypeptide consisting of a sequence selected from the group consisting of SEQ ID NOs: 29, 31, 33, and 35.
2. The polypeptide of claim 1 , wherein the polypeptide is encoded by a nucleic acid, respectively, having a sequence selected from the group consisting of SEQ ID NOs: 28, 30, 32, and 34.
3. A virus particle comprising the PRRSV-CON polypeptide of claim 1 .
4. A composition comprising the virus particle of claim 3 and a pharmaceutically acceptable carrier.
5. A composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier.
6. The composition of claim 5 , further comprising an adjuvant.
7. A method for eliciting an immune response to PRRSV in a porcine, comprising administering, to a porcine:
an effective amount of the polypeptide of claim 1 .
8. The method of claim 7 , wherein the administration is selected from the group consisting of intramuscularly, intraperitoneally, and orally.
9. A method for treating or preventing PRRS in a porcine, comprising administering, to a porcine:
an effective amount of the polypeptide of claim 1 .
10. The method of claim 9 , wherein the administration is selected from the group consisting of intramuscularly, intraperitoneally, and orally.