Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same
One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.
1. A method of recellularizing a liver of an adult subject with hepatocytes comprising:
(a) decellularizing the liver by perfusing the liver with a perfusate comprising (i) one or more oxygen carriers, (ii) a collagenase, and (iii) one or more anti-inflammatory agents to generate a decellularized liver;
(b) isolating adult cells from the perfusate; and
(c) infusing the decellularized liver with hepatocytes,
thereby recellularizing the decellularized liver with the hepatocytes.
2. The method of claim 1 , wherein the liver is a mammalian liver.
3. The method of claim 1 , wherein the liver is a human liver.
4. The method of claim 1 , wherein the adult cells are selected from the group consisting of: hepatocytes, endothelial cells, cholangiocytes (bile duct cells), Kupffer cells, stellate cells, and smooth muscle cells.
5. The method of claim 1 , wherein the liver is ischemic.
6. The method of claim 1 , wherein the one or more oxygen carriers include erythrocytes.
7. The method of claim 1 , wherein the perfusate is a normothermic perfusate.
8. The method of claim 1 , wherein the perfusate is a room temperature perfusate.
9. The method of claim 1 , wherein the perfusate is a hypothermic perfusate.
10. The method of claim 1 , further comprising culturing the isolated adult cells.
11. The method of claim 1 , wherein the step of isolating the adult cells from the perfusate includes filtering the perfusate after the perfusate has been perfused through the liver to isolate the adult cells.
12. The method of claim 11 , wherein the filtering the perfusate step comprises filtering the perfusate through one or more mesh filters to isolate the adult cells.
13. The method of claim 12 , wherein the one or more mesh filters have pore sizes ranging from about 1 μm to about 1000 μm.
14. The method of claim 1 , wherein the step of isolating the adult cells from the perfusate includes performing density centrifugation on the perfusate after the perfusate has been perfused through the liver to isolate the adult cells.
15. The method of claim 1 , further comprising circulating the perfusate through a dialyzer during step (a).
16. The method of claim 1 , wherein the liver is a damaged liver.
17. The method of claim 1 , wherein the perfusate improves the vitality of the adult cells.
18. The method of claim 1 , wherein the perfusate, as provided to the liver, further comprises one or more substrates for metabolism.
19. The method of claim 18 , wherein the one or more substrates for metabolism are selected from the group consisting of: a carbohydrate; lactate; a fatty acid; and a vitamin.
20. The method of claim 1 , wherein the perfusate, as provided to the liver, further comprises an amino acid.
21. The method of claim 19 , wherein the carbohydrate is glucose.
22. The method of claim 1 , wherein the one or more anti-inflammatory agents is selected from the group consisting of: hydrocortisone, an antihistamine, and a non-steroidal anti-inflammatory drug (NSAID).
23. The method of claim 1 , further comprising transplanting the recellularized liver into a recipient after step (c).