Vaccine for prevention of necrotic enteritis in poultry
View Patent ↗In certain embodiments, the present invention provides a poultry vaccine comprising an antigenic protein comprising a PlcC protein unit that is operably linked to a peptide linker that is operably linked to a NetB protein unit, where the vaccine is effective in stimulating a protective cellular and/or humoral immune response to C. perfringens . Methods are also provided for making the vaccine and for vaccinating poultry by administering such a vaccine.
1. An antigenic protein comprising a PlcC protein unit that is operably linked to a peptide linker that is operably linked to a NetB protein unit, wherein the PlcC protein unit, the peptide linker and the NetB protein unit each have an N-terminus and a C-terminus, wherein the C-terminus of the PlcC protein unit is linked to the N-terminus of the peptide linker, wherein the C-terminus of the peptide linker is operably linked to the N-terminus of the NetB protein unit, and wherein the peptide linker has at least 95% sequence identity to SEQ ID NO: 4.
2. The antigenic protein of claim 1 , wherein the PlcC protein unit has at least 95% sequence identity to SEQ ID NO: 3.
3. The antigenic protein of claim 1 , wherein the NetB protein unit has at least 95% sequence identity to SEQ ID NO: 5.
4. The antigenic protein of claim 3 , wherein the NetB protein unit has one or more amino acid substitutions at Y191A, R200A, W257A and W262A, S254L, R230Q or W287R of SEQ ID NO: 5.
5. The antigenic protein of claim 1 , further comprising a 6Hist tag (SEQ ID NO: 2) having an N-terminus and a C-terminus, wherein the C-terminus of the 6Hist tag is operably linked to the N-terminus of the PlcC protein unit.
6. The antigenic protein of claim 5 , further comprising a plant signal peptide having an N-terminus and a C-terminus, wherein the C-terminus of the plant signal peptide is operably linked to the N-terminus of the 6Hist tag, wherein the plant signal peptide has at least 95% sequence identity to SEQ ID NO: 1.
7. A vaccine comprising the antigenic protein of claim 1 .
8. A method of inducing an immune response against C. perfringens infection in an avian species comprising administering the vaccine of claim 7 to the avian species.
9. The method of claim 8 , wherein the vaccine is administered in poultry feed, is administered by injection, or is administered in ovo.