Methods for generating stem cell-derived beta cells and methods of use thereof
Disclosed herein are methods for generating SC-β cells, and isolated populations of SC-β cells for use in various applications, such as cell therapy.
1. A composition comprising a rho-associated protein kinase inhibitor, staurosporine, and a population of pancreatic progenitor cells that express PDX1 and NKX6.1.
2. The composition of claim 1 , wherein said rho-associated protein kinase inhibitor is selected from the group consisting of: fasudil hydrochloride, GSK269962, GSK 429286, Y-27632, and Thiazovivin.
3. The composition of claim 1 , wherein said rho-associated protein kinase inhibitor has a concentration of between about 0.1 μM and about 110 μM in said composition.
4. The composition of claim 1 , wherein said population of pancreatic progenitor cells are not genetically modified.
5. The composition of claim 1 , wherein said staurosporine has a concentration of between about 0.1 nM and about 110 nM in said composition.
6. The composition of claim 1 , wherein the composition further comprises XXI.
7. The composition of claim 6 , wherein the composition further comprises SANT1.
8. The composition of claim 7 , wherein the composition further comprises retinoic acid (RA).
9. The composition of claim 1 , wherein the composition further comprises SANT1.
10. The composition of claim 1 , wherein the composition further comprises retinoic acid (RA).
11. The composition of claim 1 , wherein the composition further comprises betacellulin.
12. The composition of claim 1 , wherein the composition further comprises T3.