Protein compositions and consumable products thereof
Provided herein are compositions with enhanced protein content, proteins with high solubility, protein combinations and methods for the preparation thereof.
1. A composition comprising a recombinant ovomucoid protein (rOVD), wherein the rOVD comprises at least one glycosylated asparagine residue and the rOVD is substantially devoid of N-linked mannosylation,
wherein the rOVD forms a clear solution at a concentration of from about 26% to about 30% w/v in an aqueous solution, at a pH of from about 2.5 to about 6, and at room temperature; and/or
wherein a solution comprising the rOVD shows substantially higher solution clarity as compared to a whey protein solution at a concentration of 9% (w/v) in an aqueous solution, at a pH of from about 2 to about 6, and at the same temperature.
2. The composition of claim 1 , wherein the composition is in powdered form.
3. The composition of claim 2 , wherein the composition has a protein content of at least 50% rOVD protein w/w.
4. The composition of claim 1 , wherein the composition comprises at least 0.1% rOVD protein w/w.
5. The composition of claim 1 , wherein rOVD comprises a polypeptide represented by an amino acid sequence selected from the group consisting of SEQ ID No. 1-44 or an amino acid sequence having at least 97% sequence identity with SEQ ID No. 1-44.
6. The composition of claim 1 , wherein rOVD is a chicken, turkey vulture, hummingbird, or goose rOVD.
7. The composition of claim 1 , wherein the rOVD is expressed by a microbial organism selected from a Pichia species, a Saccharomyces species, a Trichoderma species, a Pseudomonas species, an Aspergillus species, and an E. coli species.
8. The composition of claim 7 , wherein the microbial organism is a Pichia species.
9. A beverage composition comprising the composition of claim 1 and at least one consumable liquid, wherein the rOVD forms a clear solution in the composition, and wherein the concentration of rOVD is from about 0.1% w/v to about 30% w/v in an aqueous solution, at a pH of from about 2.5 to about 6, and at room temperature.
10. The beverage composition of claim 9 , wherein the concentration of rOVD is about 10% w/v or less or about 20% w/v or less.
11. The beverage composition of claim 10 , wherein the beverage composition is substantially optically clear.
12. The beverage composition of claim 9 , wherein the beverage composition is selected from a cold beverage, a heated beverage, and a room-temperature beverage.
13. The beverage composition of claim 9 , wherein the rOVD in the beverage composition remains substantially soluble after a heat treatment to a temperature between about 72° C. and about 121° C.
14. The beverage composition of claim 13 , wherein the heat treatment is selected from hot fill, pasteurization, and retort.
15. The beverage composition of claim 9 , wherein the beverage composition has a pH of between about 2.0 and about 6.0.
16. The beverage composition of claim 9 , wherein the rOVD is substantially a full-length protein.
17. The beverage composition of claim 9 , wherein the beverage composition is a beverage selected from a juice, a broth, a soup, a soda, a soft drink, a flavored water, a protein water, a fortified water, a carbonated water, a nutritional drink, an energy drink, a sports drink, a recovery drink, a heated drink, a coffee-based drink, a tea-based drink, a plant-based milk, a milk based drink, a non-dairy drink, plant-based milk drink, an infant formula drink, and a meal replacement drink.
18. The beverage composition of claim 9 , further comprising a second protein, wherein the rOVD and the second protein together provide a protein digestibility-corrected amino acid score (PDCAAS) of greater than about 0.85.
19. The beverage composition of claim 18 , wherein the second protein is an egg white lysozyme (OVL).
20. The beverage composition of claim 19 , wherein the ratio of rOVD to OVL is between about 60% rOVD:40% OVL and about 82% rOVD:18% OVL.