Increasing the content of long chain fatty acids in seed oil
View Patent ↗Provided are plants that express, or overexpress, a pPLAIIIδ protein. Constitutive or seed-specific expression of pPLAIIIδ protein in Arabidopsis increases seed oil content, the amount of C20 and C22 fatty acids, and the amount of C56, C58, and C60 triacylglycerols, effectively resulting in significantly higher oil yield per plant. Use of pPLAIIIδ is therefore an effective biotechnological tool to significantly increase plant yield, including oil, and the amount of high value long chain fatty acids in agricultural and horticultural crops, especially oilseed crops.
1. A transgenic oilseed plant, other than Arabidopsis, the genome of which comprises and expresses a heterologous nucleotide sequence that encodes a pPLAIIIδ protein,
wherein the pPLAIIIδ protein has at least 95% sequence similarity to the Arabidopsis pPLAIIIδ protein of SEQ ID NO: 57, and
wherein said plant is selected from the group consisting of:
(i) a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant relative to the amount of oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions;
(ii) a transgenic oilseed plant that produces oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids relative to the amount of C20 and C22 fatty acids in oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions;
(iii) a transgenic oilseed plant that produces oil in seeds of said plant containing an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of C56, C58, and C60 triacylglycerols in oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions;
(iv) a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids relative to the amount of oil and C20 and C22 fatty acids produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions;
(v) a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of oil and C56, C58, and C60 triacylglycerols produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions; and
(vi) a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids and an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of oil, C20 and C22 fatty acids, and C56, C58, and C60 triacylglycerols, respectively, produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
2. The transgenic oilseed plant of claim 1 ,
wherein said heterologous nucleotide sequence comprises Arabidopsis Genome Initiative Database Accession At3g63200 (SEQ ID NO: 58).
3. The transgenic oilseed plant of claim 1 , wherein said heterologous nucleotide sequence that encodes said pPLAIIIδ protein is expressed under the control of a seed-specific promoter.
4. The transgenic oilseed plant of claim 1 , which is selected from the group consisting of plants of the genera Brassica (e.g., rapeseed/canola ( Brassica napus; Brassica carinata; Brassica nigra; Brassica oleracea ), Camelina, Miscanthus, and Jatropha ; Jojoba ( Simmondsia chinensis ), coconut; cotton; peanut; rice; safflower; sesame; soybean; mustard other than Arabidopsis ; wheat; flax (linseed); sunflower; olive; corn; palm; palm kernel; sugarcane; castor bean; switchgrass; Borago officinalis; Echium plantagineum; Cuphea hookeriana; Cuphea pukherrima; Cuphea lanceolata; Ricinus communis; Coriandrum sativum; Crepis alpina; Vernonia galamensis; Momordica charantia ; and Crambe abyssinica.
5. The transgenic oilseed plant of claim 1 , wherein said enhanced amount of oil is about 5% higher relative to the amount of oil in seeds of a non-transformed control oilseed plant of the same species grown under the same conditions.
6. The transgenic oilseed plant of claim 1 , wherein said C20 fatty acids comprise C20:0, C20:1, C20:2, and said C22 fatty acid is C22:1.
7. The transgenic oilseed plant of claim 1 , wherein said enhanced amount of said C20 and C22 fatty acids in said oil is increased by about 10% to about 20% relative to the amount of said fatty acids in seed oil produced by a non-transformed control oilseed plant of the same species grown under the same conditions.
8. The transgenic oilseed plant of claim 1 , wherein said enhanced amount of C56, C58, and C60 triacylglycerols is about 10% to about 20% higher relative to the amount of C56, C58, and C60 triacylglycerols produced by a non-transformed control oilseed plant of the same species grown under the same conditions.
9. The transgenic oilseed plant of claim 1 , wherein said enhanced amount of oil is about 5% higher, and said enhanced amount of C20 and C22 fatty acids is about 10% to about 20% higher, relative to the amount of oil or C20 and C22 fatty acids, respectively, produced by a non-transformed control oilseed plant of the same species grown under the same conditions.
10. The transgenic oilseed plant of claim 1 , wherein said enhanced amount of oil is about 5% higher, and said enhanced amount of C56, C58, and C60 triacylglycerols is about 10% to about 20% higher, relative to the amount of oil or C56, C58, and C60 triacylglycerols, respectively, produced by a non-transformed control oilseed plant of the same species grown under the same conditions.
11. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant relative to the amount of oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
12. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids relative to the amount of C20 and C22 fatty acids in oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
13. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces oil in seeds of said plant containing an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of C56, C58, and C60 triacylglycerols in oil produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
14. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids relative to the amount of oil and C20 and C22 fatty acids produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
15. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of oil and C56, C58, and C60 triacylglycerols produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.
16. The transgenic oilseed plant of claim 1 , wherein the plant is a transgenic oilseed plant that produces an enhanced amount of oil in seeds of said plant containing an enhanced amount of C20 and C22 fatty acids and an enhanced amount of C56, C58, and C60 triacylglycerols relative to the amount of oil, C20 and C22 fatty acids, and C56, C58, and C60 triacylglycerols, respectively, produced in seeds by a non-transformed control oilseed plant of the same species grown under the same conditions.