Microfluidic trap
Provided herein are microfluidic devices that can be configured to generate an electrophoretic flow that is in opposition to a fluid flow through a microcapillary of a microfluidic device provided herein. Also provided herein are methods that include adding an amount of particle to the inlet area of a microfluidic device as provided herein, generating a first fluid flow through a microcapillary of a microfluidic device provided herein; and applying a uniform electric field to the microfluidic device, where the uniform electric field generates an electrophoretic flow that is in opposition to the fluid flow.
1. A microfluidic device consisting of:
an inlet area, where the inlet area has an entry region;
an outlet area, where the outlet area has an exit region;
a single microcapillary, where the microcapillary is fluidly coupled to the entry region of the inlet area and the exit region of the outlet area, and wherein the microcapillary is coated with a charge-neutral compound or charge-neutral polymer, wherein the inlet area, the outlet area, and the single microcapillary are formed from a single structure using laser etching;
a fluid flow generator configured to generate a fluid flow through the microcapillary;
an electric current generator configured to generate an electrophoretic flow through the microcapillary, where the electrophoretic flow is in opposition to the fluid flow, and where the electrophoretic flow is tuned to the fluid flow such that one or more types of particles present in the microcapillary migrate to a wall of the microcapillary and subsequently migrate against the fluid flow to a stagnation region,
and wherein the microfluidic device has one or more step-changes in cross-sectional area in one or more areas across a length of the device.
2. The microfluidic device of claim 1 , wherein the width of the microcapillary ranges from about 0.1 μm to about 2 mm.
3. The microfluidic device of claim 1 , wherein the length of the microcapillary ranges from about 100 μm to about 1 m.
4. The microfluidic device of claim 1 , wherein the inlet area has a height ranging from about 0.1 μm to about 500 μm and a length ranging from about 0.1 μm to about 1 m.
5. The microfluidic device of claim 1 , where the electric current generator is further configured to apply an axial electric field to the fluid.
6. The microfluidic device of claim 1 , wherein the fluid flow generator is configured to generate a fluid flow through the microcapillary at a flow velocity of 0 μm/s to 3000 μm/s.
7. A microfluidic device consisting of:
an inlet area, where the inlet area has an entry region;
an outlet area, where the outlet area has an exit region;
a single microcapillary, where the microcapillary is fluidly coupled to the entry region of the inlet area and the exit region of the outlet area, and wherein the microcapillary is coated with a charge-neutral compound or charge-neutral polymer, wherein the inlet area, the outlet area, and the single microcapillary are formed from a single structure using laser etching;
a fluid flow generator configured to generate a fluid flow through the microcapillary;
a reservoir, wherein the reservoir is fluidly coupled to the microcapillary, and wherein a capture molecule is coupled to or is physically attached to a region of the reservoir, the microcapillary, or the reservoir and microcapillary; and
an electric current generator configured to generate an electrophoretic flow through the microcapillary, where the electrophoretic flow is in opposition to the fluid flow, and where the electrophoretic flow is tuned to the fluid flow such that one or more types of particles present in the microcapillary migrate to a wall of the microcapillary and subsequently migrate against the fluid flow to a stagnation region,
and wherein the microfluidic device has one or more step-changes in cross-sectional area in one or more areas across a length of the device.