Modulating cell proliferation and pluripotency
Disclosed herein are compositions, systems, and methods for modulating proliferation, differentiation and pluripotency of cells.
1. A method for enriching a population of cells for human or mouse pluripotent stem cells, the method comprising steps of:
a) providing a population of cells comprising a mixture of pluripotent stem cells and non-pluripotent cells, wherein the pluripotent stem cells are human or mouse pluripotent stem cells; and
b) culturing the population of cells comprising the mixture of pluripotent stem cells and non-pluripotent cells in a culture medium substantially free of glutamine, wherein the culture medium comprises 0.5 mM-4 mM of cell-permeable dimethyl-α-ketoglutarate (αKG), a mitogen activated protein kinase (MAPK) inhibitor and/or a glycogen synthase kinase 3β (GSKβ) inhibitor;
wherein the pluripotent stem cells have a higher proliferation rate and/or survival rate compared to the non-pluripotent cells during said culturing, thereby producing a population in which pluripotent stem cells are enriched relative to non-pluripotent cells as compared to the provided population of cells.
2. The method of claim 1 , wherein the medium comprises the mitogen activated protein kinase (MAPK) inhibitor and the glycogen synthase kinase 3β (GSK3β) inhibitor.
3. The method of claim 1 , wherein the pluripotent stem cells are embryonic stem cells.
4. The method of claim 1 , wherein the pluripotent stem cells are induced pluripotent stem cells.
5. The method of claim 1 , wherein the provided population of cells comprises an ex vivo population of cells.
6. The method of claim 1 , wherein the produced population comprises at least 60% human or mouse pluripotent stem cells.