Gadolinium expressed lipid nanoparticles for magnetic resonance imaging
Lipid nanoparticles expressing metal ions and methods for using the compositions for magnetic resonance imaging.
1. A nanoparticle, comprising:
(a) a phospholipid selected from distearoyl-phosphocholine (DSPC) or dipalmitoyl sphingomyelin (DPSP);
(b) |N-(carbonyl-methoxypolyethyleneglycol)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (mPEG-DSPE); and
(c) 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepenta-acetyl (DSPE-DTPA) with a chelated Gd 3+ ion, wherein the nanoparticle is not a liposome, and wherein the nanoparticle has a T1 relaxivity constant (r 1 ) of at least about 133 sec −1 mM −1 as determined by a magnetic resonance (MR) scanner;
wherein the mPEG-DSPE is present in the amount from about 5 to about 20 mole percent of the combined phospholipid, the mPEG-DSPE, and DSPE-DTPA, and
wherein the phospholipid:mPEG-DSPE:DSPE-DTPA ratio is from 8:1:0.9 to 8:2:1.
2. The nanoparticle of claim 1 , wherein the N-(carbony]l-methoxypolyethyleneglycol)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (mPEG-DSPE) is N-(carbonyl-methoxypolyethyleneglycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (mPEG-2000-DSPE).
3. The nanoparticle of claim 1 , further comprising a targeting moiety.
4. The nanoparticle of claim 3 , wherein the targeting moiety is selected from the group consisting of a protein, a polypeptide, a peptide, an antibody or fragment thereof, a small molecule, a sugar or polysaccharide or derivative thereof, and a nucleic acid.
5. A composition, comprising a carrier and a plurality of the nanoparticles of claim 1 .
6. The nanoparticle of claim 1 , wherein the nanoparticle comprises 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), and N-(carbonyl-methoxypolyethyleneglycol-2000)-1,2-distearoyl-sn-glycero-3 phosphoethanolamine (mPEG-2000-DSPE).
7. The nanoparticle of claim 1 , wherein the nanoparticle does not have a solid core structure.