Carrier-binding agent compositions and methods of making and using the same
Described herein are compositions of binding agents and carrier proteins, and optionally at least one therapeutic agent, and methods of making and using the same, in particular, as a cancer therapeutic. Also described are lyophilized compositions of binding agents and carrier proteins, and optionally at least one therapeutic agent, and methods of making and using the same, in particular, as a cancer therapeutic.
1. A lyophilized nanoparticle composition comprising nanoparticle complexes having an outer surface, wherein each of the nanoparticle complexes comprises:
a) between about 100 to about 1000 binding agents arranged on the outer surface, wherein each binding agent comprises an anti-CD20 antibody, or an antigen-binding portion thereof;
b) a therapeutically effective amount of paclitaxel; and
c) albumin, wherein the albumin is non-covalently bound to paclitaxel and the binding agents;
wherein each of the nanoparticle complexes is configured such that in response to reconstitution with an aqueous solution the binding agent remains arranged on the outer surface and the anti-CD20 antibody, or antigen-binding portion thereof, of said binding agent remains capable of binding to CD20 in vivo, and wherein fewer than about 50% of said nanoparticle complexes are oligomeric.
2. The lyophilized nanoparticle composition of claim 1 , wherein the composition is stable at about 20° C. to about 25° C. for up to about 12 months or longer.
3. The lyophilized nanoparticle composition of claim 1 , wherein less than 40% of the nanoparticle complexes present in said composition are oligomerized.
4. The lyophilized nanoparticle composition of claim 1 , wherein less than 30% of the nanoparticle complexes present in said composition are oligomerized.
5. The lyophilized nanoparticle composition of claim 1 , wherein less than 20% of the nanoparticle complexes present in said composition are oligomerized.
6. The lyophilized nanoparticle composition of claim 1 , wherein less than 10% of the nanoparticle complexes present in said composition are oligomerized.
7. The lyophilized nanoparticle composition of claim 1 , wherein less than 5% of the nanoparticle complexes present in said composition are oligomerized.
8. The lyophilized nanoparticle composition of claim 1 , wherein the average size of the nanoparticle complexes is between 130 nm and 800 nm.
9. The lyophilized nanoparticle composition of claim 1 , wherein said nanoparticle complexes have an average size of approximately 160 nm.
10. The lyophilized nanoparticle composition of claim 1 , wherein the binding agents comprises one or more of, ibritumomab tiuxetan, obinutuzumab, ofatumumab, and rituximab.
11. The lyophilized nanoparticle composition of claim 1 , wherein the albumin is human serum albumin.
12. The lyophilized nanoparticle composition of claim 1 , wherein the albumin is recombinant human serum albumin.
13. The lyophilized nanoparticle composition of claim 1 , wherein upon reconstitution the composition is formulated for intravenous delivery.
14. The lyophilized nanoparticle composition of claim 13 , wherein upon reconstitution, the composition is formulated for direct injection or perfusion into a tumor.
15. The lyophilized nanoparticle composition of claim 9 , wherein a dissociation constant of albumin-bound paclitaxel and binding agent is between about 1×10 −11 M and about 1×10 −9 M.
16. A method for killing viable cancer cells in a population of cancer cells, the method comprising reconstituting the nanoparticle composition of claim 1 with an aqueous solution, and contacting the cells with a therapeutically effective amount of the reconstituted nanoparticle composition.
17. The method of claim 16 , wherein the therapeutically effective amount of the nanoparticle composition comprises about 75 mg/m 2 to about 175 mg/m 2 paclitaxel.
18. The method of claim 16 , wherein the therapeutically effective amount of the nanoparticle composition comprises about 30 mg/m 2 to about 70 mg/m 2 rituximab.