Method for magnetically labeling particles and labeling apparatus
The present disclosure relates to a method for labeling particles with magnetic particles and an apparatus for labeling particles with magnetic particles.
1. A method for labeling particles with magnetic beads, the method comprising:
supplying and feeding a specimen including the particles to a filter, the particles captured on a capture surface of the filter;
filling with liquid a flow channel on the other surface of the filter, the other surface being opposite to the capture surface,
supplying a suspension comprising magnetic beads onto the capture surface of the filter so as to create a state in which both the capture surface and the other surface of the filter are in contact with the suspension containing the magnetic beads;
from the state in which both the capture surface and the other surface of the filter are in contact with the suspension containing the magnetic beads, reversely feeding the suspension comprising the magnetic beads through the filter from the other surface of the filter toward the capture surface of the filter; and
passing the suspension comprising unreacted magnetic beads through the filter from the capture surface of the filter toward the other surface of the filter so that the unreacted magnetic beads are removed and the particles labeled with the magnetic beads are captured on the capture surface of the filter.
2. The method according to claim 1 , comprising:
stirring the suspension comprising the magnetic beads on the capture surface of the filter, after the reversely feeding.
3. The method according to claim 1 , comprising:
passing a collection liquid through the filter in a direction that is reverse of a direction in which the specimen comprising the particles passed through the filter to capture the particles, after the reversely feeding, and
collecting the collection liquid staying on the filter through suction.
4. The method according to claim 1 , comprising:
capturing the particles with the filter by passing the specimen comprising the particles through the filter at a certain flow velocity.
5. The method according to claim 4 , comprising:
passing a collection liquid through the filter in a direction that is reverse of a direction in which the specimen comprising the particles passed through the filter, after the reversely feeding, and
collecting the collection liquid staying on the filter through suction.
6. The method according to claim 4 ,
wherein the flow velocity of the specimen is 0.01 mm/sec to 100 mm/sec per opening area of the filter.
7. The method according to claim 4 ,
wherein a flow velocity at which the suspension is fed reversely is faster than 1/10 the flow velocity of the specimen.
8. The method according to claim 4 ,
wherein a flow velocity at which the suspension is fed reversely is 0.1 mm/sec or more per opening area of the filter.
9. The method according to claim 4 ,
wherein a flow velocity at which the suspension is fed reversely is faster than ⅕ the flow velocity of the specimen.
10. The method according to claim 4 ,
wherein a flow velocity at which the suspension is fed reversely is faster than ½ the flow velocity of the specimen.
11. The method according to claim 1 ,
wherein the flow velocity at which the suspension is fed reversely is 0.01 mm/sec or more per opening area of the filter.
12. The method according to claim 1 ,
wherein the particles are human or animal cells.
13. The method according to claim 1 , wherein
the magnetic beads comprise one or more of avidin, streptavidin, neutravidin, an antibody, or an antigen.