Digital PCR device and method using centrifugal force
A digital PCR device using centrifugal force. The present disclosure comprises: sample dish on which a microwell film having formed microwells is mounted; a door unit for inputting a sample while rotating the sample dish, and controlling the temperature of the sample which has been fractionated in the microwells by means of the centrifugal force and thus performing a PCR process; and a scan head unit for reading a fluorescent signal while rotating the sample which has been amplified in the microwells during the PCR process.
1. A digital PCR device using centrifugal force, the digital PCR device comprising:
a sample dish on which a microwell film including microwells formed therein is mounted, wherein the sample dish includes:
a cylinder on which the microwell film is mounted;
a cylindrical distribution cylinder provided inside the cylinder, and including grooves arranged at a regular interval such that the sample evenly spreads by the centrifugal force; and
a cover covering the cylinder;
a door unit configured to perform a PCR process by controlling a temperature of sample portions in the microwells when a sample is loaded onto the sample dish while the sample dish is rotated and is partitioned into the sample portions and loaded into the microwells by means of the centrifugal force; and
a scan head unit reading out fluorescence signals while the sample portions amplified in the microwells in the PCR process is rotated.
2. The digital PCR device of claim 1 , wherein the microwell film is mounted on an inner wall of the cylinder.
3. The digital PCR device of claim 1 , wherein the cylinder and the microwell film are made of transparent plastic or polymer.
4. The digital PCR device of claim 1 , wherein when the sample portions in the microwells are rotated, a target substance having a higher specific gravity in the sample in which the target substance and an oil component are mixed is transferred radially outward to a periphery of the sample dish, while the oil component having a lower specific gravity is transferred to a center of the sample dish, whereby the microwells are automatically sealed.
5. The digital PCR device of claim 1 , wherein the door unit includes:
a heater controlling a temperature of air;
a fan moving the air; and
a temperature sensor measuring the temperature of the air.
6. The digital PCR device of claim 5 , further comprising:
a protruded portion provided at a lower end of the door unit, and configured to spray the air in proximity to the hole of the sample dish.
7. The digital PCR device of claim 6 , wherein the protruded portion includes a nozzle spraying the air.
8. The digital PCR device of claim 5 , wherein the door unit is configured to move up and down through a hinge, and the door unit is placed and fixed to an upper portion of a support.
9. The digital PCR device of claim 8 , wherein the hinge is coupled to the support, and the support is fixed to an upper portion of a lift.
10. The digital PCR device of claim 1 , wherein the scan head unit includes a light emitting part and a light receiving part, and the scan head unit is comprised of 1 to 10 independently installed units to generate two-dimensional images and data maps.
11. The digital PCR device of claim 1 , further comprising:
a lift configured to move the sample portions amplified in the microwells in the PCR process up and down.
12. The digital PCR device of claim 11 , wherein the lift moves a supporting portion up and down to which a rotating unit rotating the sample dish is fixed.
13. The digital PCR device of claim 12 , wherein the rotating unit includes:
a mount fixing the sample dish;
a motor rotating the mount; and
an encoder controlling a rotation speed of the mount.
14. The digital PCR device of claim 11 , further comprising:
a first base fixing the lift to a first upper side thereof;
a second base on which the scan head unit is mounted; and
a support rod fixed to a second upper side of the first base to support the second base.
15. A digital PCR method using centrifugal force, the digital PCR method comprising:
mounting a sample dish to a mount and rotating the sample dish by a motor, wherein the sample dish includes:
a cylinder on which the microwell film is mounted;
a cylindrical distribution cylinder provided inside the cylinder, and including grooves arranged at a regular interval such that the sample evenly spreads by the centrifugal force; and
a cover covering the cylinder;
loading a sample onto the rotating sample dish such that the sample is partitioned into sample portions and loaded into microwells of a microwell film mounted on an inner wall of the sample dish, by means of the centrifugal force;
amplifying, by a door unit, the sample portions by performing a PCR process; and
reading out, by a scan head unit, fluorescence signals of the amplified sample portions while rotating and moving the amplified sample portions up and down.
16. The digital PCR method of claim 15 , further comprising:
generating, by the scan head unit, a two-dimensional image and a data map, by reading out the fluorescence signals of the sample portions.