Recombinant adeno-associated virus vectors to target medullary thyroid carcinoma
Provided herein are nucleic acids, recombinant adeno-associated virus (rAAV) particles, and compositions, as well as methods of use thereof for transducing medullary thyroid carcinoma cells and in treatment of disease, such as medullary thyroid carcinoma. In some aspects, the nucleic acid comprises a truncated calcitonin promoter, which is optionally encapsidated within a rAAV particle. In other aspects, the rAAV particle is a rAAV particle having a mutation in a surface-exposed amino acid, such as tyrosine, threonine, or serine, that enhances transduction of the particle into medullary thyroid carcinoma cells.
1. A nucleic acid comprising an expression construct comprising a truncated calcitonin promoter operably linked to a coding sequence of a gene of interest, wherein the truncated calcitonin promoter consists of coordinates −185 to +125 of a proximal calcitonin promoter region, relative to the transcription start site of the calcitonin coding sequence of the calcitonin gene set forth in SEQ ID NO: 3, and does not contain additional elements of a full-length calcitonin promoter, and wherein the gene of interest is CYP2B6.
2. The nucleic acid of claim 1 , wherein the expression construct is flanked on each side by an inverted terminal repeat sequence.
3. The nucleic acid of claim 1 , wherein the expression construct further comprises a tissue-specific enhancer region of the calcitonin gene having the coordinates −1080 to −860, relative to the transcription start site of the calcitonin coding sequence set forth in SEQ ID NO: 3, wherein the enhancer region is positioned 5′ of the truncated calcitonin promoter.
4. The nucleic acid of claim 1 , wherein the truncated calcitonin promoter consists of the nucleic acid sequence of SEQ ID NO: 1.
5. The nucleic acid of claim 1 , wherein expression of the gene of interest is useful to treat medullary thyroid cancer or other medullary thyroid condition or disease.
6. The nucleic acid of claim 1 , wherein the nucleic acid is a recombinant adeno-associated virus (rAAV) nucleic acid vector.
7. The nucleic acid of claim 6 , wherein the nucleic acid is a single-stranded or self-complementary rAAV nucleic acid vector.
8. A recombinant adeno-associated virus (rAAV) particle comprising the nucleic acid of claim 6 .
9. The rAAV particle of claim 8 , wherein the rAAV particle is an rAAV2 particle.
10. The rAAV particle of claim 9 , wherein the rAAV2 particle comprises a modified capsid protein comprising a non-native amino acid substitution at a position that corresponds to a surface-exposed amino acid in a wild-type AAV2 capsid protein.
11. The rAAV particle of claim 10 , wherein the non-native amino acid substitution is selected from:
(a) a non-tyrosine amino acid at Y730,
(b) a non-serine amino acid at 5662,
(c) a non-threonine amino acid at T491,
(d) a non-serine amino acid at S662 and a non-threonine amino acid at T491,
(e) a non-tyrosine amino acid at Y444, a non-tyrosine amino acid at Y500, and a non-tyrosine amino acid at Y730, or
(f) a non-tyrosine amino acid at Y444, a non-tyrosine amino acid at Y500, a non-tyrosine amino acid at Y730 and a non-threonine amino acid at T491,
in a wild-type AAV2 capsid protein (SEQ ID NO: 2).
12. The rAAV particle of claim 11 , wherein the non-native amino acid substitution is selected from:
(a) Y730F,
(b) S662V,
(c) T491V,
(d) S662V and T491V,
(e) Y444F, Y500F, and Y730F, or
(f) Y444F, Y500F, Y730F and T491V.
13. The rAAV particle of claim 12 , wherein the non-native amino acid substitution is selected from (a) Y730F and (e) Y444F, Y500F, and Y730F.
14. A composition comprising a plurality of the rAAV particle of claim 8 .
15. The composition of claim 14 , further comprising a pharmaceutically acceptable carrier.
16. A method of delivering a nucleic acid to a medullary thyroid carcinoma cell, the method comprising:
administering the nucleic acid of claim 1 to a medullary thyroid carcinoma cell.
17. The method of claim 16 , wherein the cell is a cell in a subject.
18. The nucleic acid of claim 1 , wherein the CYP2B6 gene comprises the nucleotide sequence set forth as SEQ ID NO: 4.
19. The nucleic acid of claim 3 , wherein the expression construct comprises a sequence having at least 95% sequence identity to any one of SEQ ID NOs: 8-14.
20. The nucleic acid of claim 3 , wherein the expression construct comprises the sequence of any one of SEQ ID NOs: 8-14.