Specimen processing systems and methods for holding slides
At least some embodiments of the technology are directed to an automated slide processing apparatus configured to apply at least one reagent to a specimen carried by a microscope slide. The slide processing station can include a support element with a support surface, at least one vacuum port, and a sealing member having a non-round shape. In an uncompressed state, the sealing member can extend upwardly beyond the support surface. In a compressed state, the sealing member can be configured to maintain an airtight seal with a backside of the microscope slide as the microscope slide is pulled against the support surface by a vacuum drawn via the at least one vacuum port.
1. A method for holding a microscope slide, comprising:
positioning a microscope slide on a support element such that a specimen-bearing portion of the slide is supported by a first portion of the support element and a label-bearing portion of the slide is supported by a second portion of the support element, wherein the first portion surrounds the second portion and a sealing member is positioned between the first and the second portions;
forming a vacuum chamber between the second portion and the backside of the microscope slide; and
drawing a vacuum through the second portion supporting the label-bearing portion while the sealing member contacts a backside of the slide, wherein drawing the vacuum includes drawing a sufficient vacuum to form an airtight seal between the sealing member and the slide to inhibit at least one of translational or rotational movement of the slide relative to the support element.
2. The method of claim 1 , wherein positioning the microscope slide includes contacting a top portion of the sealing member such that the top portion of the sealing member deflects in a direction that is generally perpendicular to a backside surface of the slide.
3. The method of claim 1 , wherein the first portion, the second portion, and the sealing member each contact a backside of the microscope slide while drawing the vacuum.
4. A method of dispensing liquid onto a microscope slide, the method comprising:
sequentially delivering reagents to a plurality of reservoir wells of a carousel to produce reagent mixtures, wherein the carousel is rotatable to sequentially position the reservoir wells at a position under a reagent pipette;
at least partially filling a reagent pipette with one of the reagent mixtures from one of the reservoir wells while at least one of the reservoir wells is located at a position under a wash pipette;
robotically moving the reagent pipette, carrying the reagent mixture, toward the microscope slide and dispensing the reagent mixture from the reagent pipette onto the microscope slide; and
washing at least one of the reservoir wells at the same time as mixing reagents when the at least one reservoir well is located at the position under the wash pipette and the reagent mixture is located in the at least one of the reservoir wells.
5. A method of transporting specimen-bearing microscope slides in an automated processing system, comprising:
sequentially moving a plurality of specimen-bearing microscope slides from a carrier to a slide staging device one at a time;
aligning the individual specimen-bearing microscope slides one at a time along a longitudinal axis at the slide staging device to an aligned position by the slide staging device;
contacting opposing sides of the individual specimen-bearing microscope slide with pivotable aligning members of the slide staging device while the bottom surface overlays a sealing member of the slide staging device, wherein the sealing member is configured to sealingly contact the bottom surface, and wherein the slide staging device is configured to draw a vacuum to hold a bottom surface of the individual specimen-bearing microscope slide; and
transporting the individual specimen-bearing microscope slides from the slide staging device to one or more specimen processing stations while in the aligned position.
6. The method of claim 5 , further comprising alternatingly aligning and transporting the individual specimen-bearing microscope slides to sequentially load the specimen processing stations with the individual specimen-bearing microscope slides.