Method for promoting growth and bioactive substances of
A method for promoting growth and bioactive substances of Crepidiastrum denticulatum including performing a stress treatment on Crepidiastrum denticulatum during cultivation thereof, in which the stress treatment includes at least of applying visible light, drying, exposing to low temperature, irradiating ultraviolet rays, and applying a chemical elicitor.
1. A method for promoting growth and bioactive substances of Crepidiastrum denticulatum , the method comprising:
performing a stress treatment on Crepidiastrum denticulatum during cultivation thereof, the stress treatment comprising at least one of applying visible light, drying, exposing to low temperature, irradiating ultraviolet rays, and applying a chemical elicitor.
2. The method of claim 1 , wherein the stress treatment comprises applying visible light, the visible light is at least one of white light, red light, green light, and blue light.
3. The method of claim 2 , wherein the visible light is one monochromatic light of red light, green light, and blue light.
4. The method of claim 2 , wherein the visible light comprises mixed light having the ratio of at least one of:
red light:blue light=6:4; red light:blue light=7:3; red light:blue light=8:2; red light:blue light=9:1; red light:green light:blue light=5:1:4; red light:green light:blue light=6:1:3; red light:green light:blue light=7:1:2; red light:green light:blue light=8:1:1; red light:green light:blue light=9:1:0; red light:green light:blue light=7:2:1; red light:white light:blue light=8:1:1; red light:white light:blue light=6:2:2; red light:white light:blue light=7:1:2; and red light:white light:blue light=8:2:0.
5. The method of claim 4 , wherein the mixed light further comprises a far-red light.
6. The method of claim 5 , wherein the mixed light comprises a red light and a blue light mixed at a ratio of 8:2.
7. The method of claim 6 , wherein a ratio of the red light to the far-red light is at least one of 1.7, 1.2, 4.1, and 8.6 to 1.
8. The method of claim 5 , further comprising harvesting Crepidiastrum denticulatum on the 6th week after planting Crepidiastrum denticulatum , when the mixed light comprising the far red light is applied during cultivation.
9. The method of claim 1 , further comprising harvesting Crepidiastrum denticulatum on the 6th week after planting Crepidiastrum denticulatum , when the visible light is applied during cultivation.
10. The method of claim 1 , wherein the stress treatment comprises drying, the drying comprises stop watering Crepidiastrum denticulatum for a predetermined period of time.
11. The method of claim 10 , further comprising harvesting Crepidiastrum denticulatum on the 3 rd to 5 th days after the drying stress treatment.
12. The method of claim 1 , wherein the stress treatment comprises drying, the drying comprises supplying water to Crepidiastrum denticulatum for a predetermined period of time using a wick.
13. The method of claim 12 , further comprising harvesting Crepidiastrum denticulatum on the 2 nd day after the drying stress treatment.
14. The method of claim 1 , wherein the stress treatment comprises exposing to low temperature, the low temperature stress treatment comprises exposing Crepidiastrum denticulatum at 10° C. during one of night time and day time.
15. The method of claim 1 , wherein the stress treatment comprises exposing to low temperature, further comprising harvesting Crepidiastrum denticulatum on the 3 rd to 5 th days after the low temperature stress treatment.
16. The method of claim 1 , wherein the stress treatment comprises irradiating ultraviolet rays, the ultraviolet rays comprises UV.
17. The method of claim 16 , further comprising harvesting Crepidiastrum denticulatum 8 hours after UV-A is irradiated to Crepidiastrum denticulatum.
18. The method of claim 1 , wherein the stress treatment comprises irradiating ultraviolet rays, the ultraviolet rays comprises UV-B.
19. The method of claim 18 , further comprising harvesting Crepidiastrum denticulatum on the 2 nd day after UV-B is irradiated to Crepidiastrum denticulatum.
20. The method of claim 19 , wherein UV-B is irradiated to Crepidiastrum denticulatum for 1 hour in every 11 hours during 2 days.
21. The method of claim 1 , wherein the stress treatment comprises applying the chemical elicitor, the chemical elicitor comprises salicylic acid.
22. The method of claim 21 , further comprising harvesting Crepidiastrum denticulatum on the 3 rd day after the chemical elicitor is sprayed onto Crepidiastrum denticulatum.
23. The method of claim 1 , wherein Crepidiastrum denticulatum after the stress treatment has an increase in at least one of a shoot fresh weight, a shoot dry weight, number of leaves, a leaf area, a leaf length, and a leaf width of an aerial part.
24. The method of claim 1 , wherein the bioactive substances of Crepidiastrum denticulatum promoted by the stress treatment comprise a phenolic compound and a chicoric acid.