Process for producing extracted lipid comprising docosahexaenoic acid
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
1. A process for producing extracted lipid, comprising the steps of:
a) obtaining Brassica napus or Camelina sativa seed comprising extractable lipid having a total fatty acid content, the lipid comprising fatty acids esterified as triacylglycerols (TAG), the fatty acids comprising oleic acid, palmitic acid, linoleic acid (LA), γ-linolenic acid (GLA), α-linolenic acid (ALA), stearidonic acid (SDA), docosapentaenoic acid (DPA), docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and eicosatetraenoic acid (ETA), wherein at least 70% by weight of the DHA which is esterified in the form of TAG is esterified at the sn-1 or sn-3 position of TAG, and
b) extracting lipid from the seed.
2. The process of claim 1 , wherein EPA, DPA and DHA, and optionally arachidonic acid (ARA), are each present at a level in the total fatty acid content, each level being expressed as a percentage of the total fatty acid content, whereby the sum total of the levels of ARA, EPA, DPA and DHA in the extractable lipid is between 7% and 25% by weight of the total fatty acid content.
3. The process of claim 1 , wherein GLA is present in the extracted lipid at a level of less than 2% by weight of the total fatty acid content.
4. The process of claim 3 , wherein at least 80% by weight of the DHA esterified in the form of TAG in the extracted lipid is at the sn-1 or sn-3 position of TAG.
5. The process of claim 1 , wherein SDA, ETA, EPA, DPA and DHA are each present at a level in the total fatty acid content, each level being expressed as a percentage by weight of the total fatty acid content, whereby the sum of the percentages for ETA, EPA, DPA and DHA divided by the sum of the percentages for SDA, ETA, EPA, DPA and DHA, expressed as a percentage, is at least 75%.
6. The process of claim 5 , wherein at least 80% by weight of the DHA esterified in the form of TAG in the extracted lipid is at the sn-1 or sn-3 position of TAG.
7. The process of claim 1 , wherein ETA, EPA, DPA and DHA are each present at a level in the total fatty acid content, each level being expressed as a percentage by weight of the total fatty acid content, whereby the sum of the percentages for EPA, DPA and DHA divided by the sum of the percentages for ETA, EPA, DPA and DHA, expressed as a percentage, is between 75% and 98%.
8. The process of claim 7 , wherein at least 80% by weight of the DHA esterified in the form of TAG in the extracted lipid is at the sn-1 or sn-3 position of TAG.
9. The process of claim 1 , wherein DHA is present in the extracted lipid at a level of between 2.7% and 3.3% by weight of the total fatty acid content.
10. The process of claim 1 , wherein DHA is present in the extracted lipid at a level of between 7% and 20% by weight of the total fatty acid content.
11. The process of claim 1 , further comprising a step of purifying the extracted lipid.
12. The process of claim 2 , further comprising a step of purifying the extracted lipid.
13. The process of claim 3 , further comprising a step of purifying the extracted lipid.
14. The process of claim 4 , further comprising a step of purifying the extracted lipid.
15. The process of claim 1 , further comprising a step of transesterifying the extracted lipid to thereby produce fatty acid esters.
16. The process of claim 15 , further comprising a step of fractionating the fatty acid esters.
17. The process of claim 1 , wherein the Brassica napus or Camelina sativa seeds of step a) are obtained from Brassica napus or Camelina sativa plants grown in a field as a population of at least 1,000 plants.
18. The process of claim 17 , wherein the process comprises, prior to step a), a step of cultivating the at least 1000 Brassica napus or Camelina sativa plants in the field, and step a) comprises harvesting Brassica napus or Camelina sativa seeds from the at least 1000 Brassica napus or Camelina sativa plants.