Process for production of Galacto-oligosaccharides
A process for production of galacto-oligosaccharides is provided. The process for production of galacto-oligosaccharides includes growing Sporobolomyces singularis in a fermentation medium, harvesting the said microbes from the fermentation medium, inoculating the said microbes with lactose solution in a reactor and incubating in an aerobic condition to convert lactose into galacto-oligosaccharides. The process also includes separating the galacto-oligosaccharides from the microbes; filtering thus obtained galacto-oligosaccharides; and optionally making the powder form of galacto-oligosaccharides. The process for producing galacto-oligosaccharides provides high yield and high purity.
1. A process for producing galacto-oligosaccharides comprising:
(a) growing Sporobolomyces singularis MCC 0096 microbes in a fermentation medium;
(b) harvesting the microbes;
(c) inoculating the harvested microbes with lactose solution in a reactor in an aerobic condition, at a temperature in the range of 35 to 40° C., wherein the ratio of the said microbes with respect to lactose solution is at least 5% on w/v basis;
(d) separating the galacto-oligosaccharides from the microbes;
(e) filtering the obtained galacto-oligosaccharides of step (d);
(f) optionally making a powder form of galacto-oligosaccharides, wherein the microbes convert at least 80% of lactose into galacto-oligosaccharides.
2. The process of claim 1 , wherein the microbes are harvested from the fermentation medium at the OD 620 range of 5.0-6.0.
3. The process of claim 1 , wherein microbes are grown in the fermentation medium at a temperature range of 25 to 30° C. in an aerobic condition.
4. The process of claim 1 , wherein the separation is carried out using a microfiltration system and/or centrifugation.
5. The process of claim 1 , wherein the powder is made by at least one of spray drying and crystallization.
6. The process of claim 1 , wherein the yield of galacto-oligosaccharides is at least 80%.
7. The process of claim 1 , wherein the microbes are reused.