IP Library › Granted Patent US 11,321,836
Granted Patent B2
US 11,321,836 · App. 16/838,950 · Granted May 3, 2022

Image-processing device, image-processing method, and image-processing program for setting cell analysis area based on captured image

Inventors: Mamiko Masutani (Yokohama, JP); Kenta Imai (Chigasaki, JP); Wataru Tomosugi (Yokohama, JP); Takuro Saigo (Tokyo, JP); Chisako Iwamoto (Yokohama, JP); Masafumi Yamashita (Fujisawa, JP); Kenichi Ohba (Tokyo, JP); Masayuki Murata (Tokyo, JP); Fumi Kano (Tokyo, JP); Yoshiyuki Noguchi (Tokyo, JP)
Assignees: NIKON CORPORATION; THE UNIVERSITY OF TOKYO
G06T7/0012G01N15/1475G06T2207/30024G06T2207/30242
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Quick Facts
Patent No.
US 11,321,836
App. No.
16/838,950
Granted
May 3, 2022
Kind
B2
Abstract

An image-processing device including: a determination unit that determines a captured image on the basis of a ratio of cells and a distribution of the cells in the captured image acquired by imaging a sample including cells; and an area setting unit that sets an area used for analyzing the cells of the sample on the basis of a result of the determination acquired by the determination unit.

Claims (28)

1. An image-processing device comprising:

a processor programed to

a determination unit configured to determine perform a determination with respect to a captured image on the basis of (a) at least one selected from the group of the number of cells and a ratio of cell area and (b) a distribution of the cells in the captured image acquired by imaging a sample including a plurality of cells; and

set an area used for analyzing the cells on the basis of a result of the determination.

2. The image-processing device according to claim 1 , wherein the processor is programed to calculate (a) at least one selected from the group of the number of cells and the ratio of cell area and (b) the distribution of the cells on the basis of cell nucleuses in the cells imaged in the captured image.

3. The image-processing device according to claim 1 , wherein resolution of the captured image is lower than that of an image used for the analysis of the cells.

4. The image-processing device according to claim 1 , wherein the processor is programed to calculate (a) at least one selected from the group of the number of cells and the ratio of cell area and (b) the distribution of the cells by determining whether or not the cells are at predetermined positions in the captured image.

5. The image-processing device according to claim 4 , wherein the predetermined positions are positions at which a plurality of straight lines set at a predetermined interval in a first direction of the captured image and a plurality of straight lines set at the predetermined interval in a vertical direction to the first direction of the captured image intersect with each other.

6. The image-processing device according to claim 5 , wherein the predetermined interval is approximately half of a length of a diameter of the cell nucleuses in the captured image.

7. The image-processing device according to claim 1 , wherein the processor is programed to calculate (a) at least one selected from the group of the number of cells and the ratio of cell area and (b) the distribution of the cells on the basis of shapes of constituent elements of the cells in the captured image.

8. The image-processing device according to claim 1 , wherein

the captured image is a captured image captured with an imaging magnification lower than a magnification of an image used for the analysis, and

the processor is further programed to select candidates for an area in which an image used for the analysis is captured by calculating, for the captured image, (a) at least one selected from the group of the number of cells and the ratio of cell area and (b) the distribution of the cells for each imaging range according to an imaging magnification of the image used for the analysis.

9. The image-processing device according to claim 1 , wherein the processor is further programed to acquire a plurality of the captured images in different fields of vision.

10. The image-processing device according to claim 9 , wherein the processor is programed to receive the captured image from another device capturing the captured image.

11. The image-processing device according to claim 9 ,

wherein the captured image is captured through an objective lens, and

wherein the different fields of vision are different along an optical axis of the objective lens.

12. The image-processing device according to claim 9 ,

wherein the captured image is captured through an objective lens, and

wherein the different fields of vision are different along a plane that is orthogonal to an optical axis of the objective lens.

13. The image-processing device according to claim 1 , wherein the processor is programed to determine whether or not the captured image is appropriate for the analysis of the cells.

14. An image-processing method comprising:

determining a captured image on the basis of (a) at least one selected from the group of the number of cells and a ratio of cell area and (b) a distribution of the cells in the captured image acquired by imaging a sample including a plurality of cells; and

setting an area used for analyzing the cells on the basis of a result of the determination acquired in the determining of the captured image.

15. A non-transitory computer readable medium storing thereon an image-processing program causing a computer to execute:

determining a captured image on the basis of (a) at least one selected from the group of the number of cells and a ratio of cell area and (b) a distribution of the cells in the captured image acquired by imaging a sample including a plurality of cells; and

setting an area used for analyzing the cells on the basis of a result of the determination acquired in the determining of the captured image.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 23, 2021
From: NIKON CORPORATION
To: UNIVERSITY OF TOKYO
Reel/Frame 057256/0901 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 23, 2021
From: MURATA, MASAYUKI; KANO, FUMI; NOGUCHI, YOSHIYUKI
To: NIKON CORPORATION; THE UNIVERSITY OF TOKYO
Reel/Frame 057257/0255 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 2, 2020
From: MASUTANI, MAMIKO; IMAI, KENTA; TOMOSUGI, WATARU; SAIGO, TAKURO; IWAMOTO, CHISAKO; YAMASHITA, MASAFUMI; OHBA, KENICHI
To: NIKON CORPORATION
Reel/Frame 052301/0313 →
Continuity (2)
Continuation PCTJP2017036442 · Oct 6, 2017
Related Publication 20210073979A1 · Mar 11, 2021