Coated silk films, methods for the production thereof and uses thereof
The present invention relates to coated silk films. Further, the present invention relates to pharmaceutical or cosmetic compositions comprising the coated silk films. Furthermore, the present invention relates to coated silk films or pharmaceutical compositions comprising the coated silk films for use in medicine. In addition, the present invention relates to methods of producing the coated silk films.
1. A coated silk film consisting of
(i) one silk film layer comprising at least one active agent with a molecular weight between 50 Da and 150 kDa and having a top surface, a bottom surface, and two side surfaces; and
(ii) one release modifying layer completely and continuously covering all surfaces of the silk film layer,
wherein the coated silk film is made by first casting a silk film layer with a first silk polypeptide solution comprising the at least one active agent and drying the silk film layer, and then coating the silk film layer with a second silk polypeptide solution to form a release modifying layer completely and continuously covering all surfaces of the silk film layer, wherein each of the first and second silk polypeptide solutions is a 1-10% w/v silk polypeptide solution, and wherein each of the first and second silk polypeptides is C 16 .
2. The coated silk film of claim 1 , wherein the least one active agent has a molecular weight between 50 kDa and 150 kDa.
3. The coated silk film of claim 1 , wherein the first silk polypeptide solution is a 2.5% w/v silk polypeptide solution, and the second silk polypeptide solution is a 5% w/v silk polypeptide solution.
4. The coated silk film of claim 1 , wherein the active agent is selected from the group consisting of a biological agent, a pharmaceutical agent, a cosmetic agent, a nutrient, and a dietary supplement.
5. A method of producing a coated silk film comprising the steps of:
(i) casting a silk film layer with a first silk polypeptide solution comprising at least one active agent with a molecular weight between 50 Da and 150 kDa and drying the silk film layer to provide one silk film layer comprising the at least one active agent and having a top surface, a bottom surface, and two side surfaces; and
(ii) dipping the silk film layer in a second silk polypeptide solution to form a release modifying layer completely and continuously covering all surfaces of the silk film layer,
wherein each of the first and second silk polypeptide solutions is a 1-10% w/v silk polypeptide solution, and wherein each of the first and second silk polypeptides is C 16 .
6. The method of claim 5 , wherein the least one active agent has a molecular weight between 50 kDa and 150 kDa.
7. The method of claim 5 , wherein the first silk polypeptide solution is a 2.5% w/v silk polypeptide solution, and the second silk polypeptide solution is a 5% w/v silk polypeptide solution.
8. The method of claim 5 , wherein the method further comprises the step of drying the release modifying layer.
9. The method of claim 5 , wherein the method further comprises the step of applying pressure to the release modifying layer.
10. The method of claim 5 , wherein the method further comprises the step of applying water vapour to the release modifying layer.