Modified AAV capsid proteins and uses thereof

1. A variant recombinant adeno-associated virus (rAAV) capsid protein comprising any one of the following combinations of amino acid substitutions:

(a) phenylalanine (F), serine (S), aspartic acid (D), isoleucine (I), aspartic acid (D), asparagine (N), methionine (M), alanine (A), aspartic acid (D), phenylalanine (F), aspartic acid (D), and glycine (G) at positions corresponding to amino acids 444, 450, 451, 454, 455, 459, 461, 492, 499, 500, 530, and 531 of AAV2 VP1 capsid protein, respectively;

(b) phenylalanine (F), aspartic acid (D), serine (S), methionine (M), threonine (T), arginine (R), valine (V), phenylalanine (F), and aspartic acid (D) at positions corresponding to amino acids 444, 450, 454, 457, 459, 461, 491, 500, and 531 of AAV2 VP1 capsid protein, respectively;

(c) asparagine (N), alanine (A), aspartic acid (D), glycine (G), glutamic acid (E), aspartic acid (D), phenylalanine (F), and glutamic acid (E) at positions corresponding to amino acids 263, 264, 492, 493, 494, 499, 500, and 545 of AAV2 VP1 capsid protein, respectively;

(d) asparagine (N), alanine (A), aspartic acid (D), glycine (G), glutamic acid (E), aspartic acid (D), and phenylalanine (F) at positions corresponding to amino acids 263, 264, 492, 493, 494, 499, and 500 of AAV2 VP1 capsid protein, respectively;

(e) phenylalanine (F), threonine (T), arginine (R), aspartic acid (D), and arginine (R) at positions corresponding to amino acids 500, 507, 527, 530, and 532 of AAV2 VP1 capsid protein, respectively; or

(f) glutamine (Q), aspartic acid (D), glutamic acid (E), phenylalanine (F), and proline (P) at positions corresponding to amino acids 491, 492, 494, 500, and 503 of AAV2 VP1 capsid protein, respectively, and SAAGADXAXDS (SEQ ID NO: 5) at positions corresponding to amino acids 546-556 of AAV2 VP1 capsid protein;

wherein each X corresponds to amino acids of a wild-type AAV2 VP1 capsid sequence as set forth in SEQ ID NO: 1, or homologous amino acids of a wild-type VP1 capsid sequence of an AAV serotype other than AAV2.

2. The rAAV capsid protein of claim 1 , comprising (a) phenylalanine (F), serine (S), aspartic acid (D), isoleucine (I), aspartic acid (D), asparagine (N), methionine (M), alanine (A), aspartic acid (D), phenylalanine (F), aspartic acid (D), and glycine (G) at positions corresponding to amino acids 444, 450, 451, 454, 455, 459, 461, 492, 499, 500, 530, and 531 of AAV2 VP1 capsid protein, respectively.

3. The rAAV capsid protein of claim 2 further comprising asparagine (N) and alanine (A) at positions corresponding to amino acids 263 and 264 of AAV2 VP1 capsid protein, respectively.

4. The rAAV capsid protein of claim 1 , comprising (b) phenylalanine (F), aspartic acid (D), serine (S), methionine (M), threonine (T), arginine (R), valine (V), phenylalanine (F), and aspartic acid (D) at positions corresponding to amino acids 444, 450, 454, 457, 459, 461, 491, 500, and 531 of AAV2 VP1 capsid protein, respectively.

5. The rAAV capsid protein of claim 4 further comprising alanine (A) at positions corresponding to amino acids 263 and 264 of AAV2 VP1 capsid protein.

6. The rAAV capsid protein of claim 4 further comprising aspartic acid (D), glycine (G), and glutamic acid (E) at positions corresponding to amino acids 492, 493, and 494 of AAV2 VP1 capsid protein, respectively.

7. The rAAV capsid protein of claim 6 further comprising aspartic acid (D) at amino acid position 499 of AAV2 VP1 capsid protein.

8. The rAAV capsid protein of claim 1 , comprising (c) asparagine (N), alanine (A), aspartic acid (D), glycine (G), glutamic acid (E), aspartic acid (D), phenylalanine (F), and glutamic acid (E) at positions corresponding to amino acids 263, 264, 492, 493, 494, 499, 500, and 545 of AAV2 VP1 capsid protein, respectively.

9. The rAAV capsid protein of claim 1 , comprising (d) asparagine (N), alanine (A), aspartic acid (D), glycine (G), glutamic acid (E), aspartic acid (D), and phenylalanine (F) at positions corresponding to amino acids 263, 264, 492, 493, 494, 499, and 500 of AAV2 VP1 capsid protein, respectively.

10. The rAAV capsid protein of claim 1 , comprising (e) phenylalanine (F), threonine (T), arginine (R), aspartic acid (D), and arginine (R) at positions corresponding to amino acids 500, 507, 527, 530, and 532 of AAV2 VP1 capsid protein, respectively.

11. The rAAV capsid protein of claim 10 further comprising glycine (G) and threonine (T) at positions corresponding to amino acids 263 and 264 of AAV2 VP1 capsid protein, respectively.

12. The rAAV capsid protein of claim 1 , comprising (f) glutamine (Q), aspartic acid (D), glutamic acid (E), phenylalanine (F), and proline (P) at positions corresponding to amino acids 491, 492, 494, 500, and 503 of AAV2 VP1 capsid protein, respectively, and SAAGADXAXDS (SEQ ID NO: 5) at positions corresponding to amino acids 546-556 of AAV2 VP1 capsid protein.

13. The rAAV capsid protein of claim 12 further comprising asparagine (N) and alanine (A) at positions corresponding to amino acids 263 and 264 of AAV2 VP1 capsid protein, respectively.

14. A variant rAAV capsid protein of a serotype other than serotype 2 comprising a combination of amino acid substitutions in accordance with claim 1 , wherein the substitutions are positioned in a variable region of the capsid protein that is homologous to the variable region of AAV2.

15. The variant rAAV capsid protein of a serotype other than serotype 2 of claim 14 , wherein the capsid protein is of serotype 1, 3, 3B, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13.

16. A recombinant AAV (rAAV) particle comprising the rAAV capsid protein of claim 1 .

17. The rAAV particle of claim 16 , wherein the particle is an AAV2 particle.

18. The rAAV particle of claim 17 further comprising a nucleic acid comprising inverted terminal repeats (ITRs) and a gene of interest.

19. The rAAV particle of claim 18 , wherein the nucleic acid is single stranded or double-stranded.

20. A composition comprising a plurality of the variant recombinant AAV2 particles of claim 16 .