Composite materials comprising synthetic dragline spider silk
Composites based on a polymer and a mixture of proteins derived from a MaSp (major ampullate spidroin) protein are provides. Further, methods for preparation of same, and method of use of the composites are provided.
1. A composite comprising a mixture of proteins in the form of a fiber and at least one polymer, wherein: said mixture of proteins comprises between 4 and 32 types of proteins of differing molecular weight and a different number of repeats of a repetitive sequence, wherein each protein in said mixture comprises, independently, 2 to 70 repeats of said repetitive sequence, said repetitive sequence comprises an amino acid sequence as set forth in SEQ ID NO: 1:
(X 1 ) Z X 2 GPGGYGPX 3 X 4 X 5 GPX 6 GX 7 GGX 8 GPGGPGX 9 X 10 ,
wherein X 1 is, independently, at each instance A or G wherein at least 50% of (X 1 )Z is A, Z is an integer between 5 to 30; X 2 is S or G; X 3 is G or E; X 4 is G, S or N; X 5 is Q or Y; X 6 is G or S; X 7 is P or R; X 8 is Y or Q; X 9 is G or S; and X 10 is S or G, wherein said mixture is characterized by molecular weight increment of 2 kDa to 3.5 kDa, between at least two proteins in said mixture.
2. The composite of claim 1 , wherein said mixture of proteins is characterized by each repeat having a molecular weight in the range of 2 kDa to 3.5 kDa.
3. The composite of claim 1 , wherein said polymer is a synthetic polymer, a thermoplastic polymer, a thermoset, an epoxy, a polyester, a polyamide, a polyol, a polyurethane, polyethylene, Nylon, a polyacrylate, a polycarbonate, a polylactic acid (PLA) or a copolymer thereof, a silicon, a liquid crystal polymer, a maleic anhydride grafted polypropylene, polycaprolactone (PCL), rubber, cellulose, or any combination thereof, optionally wherein at least one polymer is a non MaSp protein derived polymer.
4. The composite of claim 1 , wherein said repetitive sequence comprises the amino acid sequence as set forth in SEQ ID NO: 3 (AAAAAAAASGPGGYGPGSQGPSGPGGYGPGGPGSS).
5. The composite of claim 1 , characterized by (i) at least one improved mechanical property as compared to the property for the polymer free of said mixture of proteins, wherein said property is selected from the group consisting of: Young's modulus, tensile strength, fracture strain, yield point, toughness, work to failure, impact strength, tear strength, flexural modulus, flexural strain and stress at a specific percentage elongation or (ii) being comprised within a layer of at least 0.5 micron thick.
6. The composite of claim 1 , wherein a total concentration of said proteins ranges from about 0.1 weight percent to about 3 weight percent of the total weight of said composite.
7. An article comprising the composite of claim 1 .
8. The article of claim 7 , comprising a biocompatible material or an implantable material.
9. A pharmaceutical composition, comprising the composite of claim 1 and a pharmaceutically acceptable carrier.