Restimulation of cryopreserved tumor infiltrating lymphocytes
The present disclosure provides methods for re-stimulating TIL populations that lead to improved phenotype and increased metabolic health of the TILs and provides methods of assaying for TIL populations to determine suitability for more efficacious infusion after re-stimulation.
1. A cryopreserved tumor infiltrating lymphocyte (TIL) composition comprising a therapeutic population of TILs, wherein the cryopreserved TIL composition is produced by the method comprising:
(a) performing a first expansion by (i) thawing cryopreserved dissociated tumor materials comprising a first population of TILs obtained from a tumor that was resected from a subject, and (ii) culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs;
(b) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, and wherein the second expansion is performed for about 7 to 14 days in order to obtain the third population of TILs;
(c) harvesting the third population of TILs obtained from step (b);
(d) transferring the harvested third population of TILs from step (c) into an infusion bag; and
(e) cryopreserving the infusion bag comprising the harvested TIL population from step (d) using a cryopreservation process.
2. The cryopreserved TIL composition according to claim 1 , wherein the dissociated tumor materials comprise a tumor digest.
3. The cryopreserved TIL composition according to claim 1 , wherein obtaining the dissociated tumor materials comprises fragmenting the tumor resected from the subject into one or more tumor fragments.
4. The cryopreserved TIL composition according to claim 1 , wherein obtaining the dissociated tumor materials comprises mechanically disrupting the tumor resected from the subject.
5. The cryopreserved TIL composition according to claim 1 , wherein obtaining the dissociated tumor materials comprises enzymatically disrupting the tumor resected from the subject.
6. The cryopreserved TIL composition according to claim 1 , wherein the cell culture medium is CTS Optimizer.
7. The cryopreserved TIL composition according to claim 1 , wherein the third population of TILs harvested in step (c) comprises sufficient TILs for use in administering a therapeutically effective dosage to a subject.
8. The cryopreserved TIL composition according to claim 7 , wherein the therapeutically effective dosage of TILs comprises from about 1×10 9 to about 9×10 10 TILs.
9. The cryopreserved TIL composition according to claim 1 , wherein the APCs comprise peripheral blood mononuclear cells (PBMCs).
10. The cryopreserved TIL composition according to claim 1 , wherein the first expansion is performed within from about 3 to 11 days.
11. The cryopreserved TIL composition according to claim 1 , wherein steps (a) through (e) are performed within about 24 days.
12. The cryopreserved TIL composition according to claim 1 , wherein step (a) further comprises adding the thawed dissociated tumor materials into a closed system prior to culturing the first population of TILs.
13. The cryopreserved TIL composition according to claim 12 , wherein the transition from step (a) to step (b), the transition from step (b) to step (c), or the transition from step (c) to step (d) occurs without opening the system.
14. The cryopreserved TIL composition according to claim 13 , wherein the transition from step (a) to step (b) occurs without opening the system.
15. The cryopreserved TIL composition according to claim 13 , wherein the transition from step (b) to step (c) occurs without opening the system.
16. The cryopreserved TIL composition according to claim 13 , wherein the transition from step (c) to step (d) occurs without opening the system.
17. The cryopreserved TIL composition according to claim 1 , wherein step (a) further comprises adding the thawed dissociated tumor materials into a closed system prior to culturing the first population of TILs, wherein the transition from step (a) to step (b) occurs without opening the system, wherein the transition from step (b) to step (c) occurs without opening the system, and wherein the transition from step (c) to step (d) occurs without opening the system.
18. The cryopreserved TIL composition according to claim 1 , wherein the cryopreserved dissociated tumor materials were obtained by cryopreserving dissociated tumor materials in a cryopreservation media.
19. The cryopreserved TIL composition according to claim 18 , wherein the cryopreservation media comprises DMSO.
20. The cryopreserved TIL composition according to claim 18 , wherein the cryopreservation media comprises 5% DMSO.