Methods and compositions for PDGF-CC inhibition
The present invention relates, in part, to isolated antibodies that specifically interact with and show measurable binding affinity to an epitope of platelet derived growth factor C (PDGF-C). Such antibodies may be used for the modulation of PDGF-C activity in or secreted from a cell to study its effects on cell function and, in certain embodiments, for the treatment and/or prevention of a disease or condition associated with PDGF-C signing pathway.
1. An isolated antibody or antigen-binding fragment thereof that specifically interacts and shows measurable affinity to a polypeptide comprising the active portion of PDGF-C (SEQ ID NO: 2) or a polypeptide having at least 90% sequence identity thereto, wherein said antibody comprises:
(a) light chain complementary determining regions CDR1L, CDR2L, and CDR3L, wherein CDR1L comprises SEQ ID NO: 16; CDR2L comprises SEQ ID NO: 17; and CDR3L comprises SEQ ID NO: 18; and
(b) heavy chain complementary determining regions CDR1H, CDR2H, and CDR3H, wherein:
(i) CDR1H comprises SEQ ID NO: 34, CDR2H comprises SEQ ID NO: 35, and CDR3H comprises SEQ ID NO: 36;
(ii) CDR1H comprises SEQ ID NO: 46, CDR2H comprises SEQ ID NO: 47, and CDR3H comprises SEQ ID NO: 48; or
(iii) CDR1H comprises SEQ ID NO: 49, CDR2L comprises SEQ ID NO: 50, and CDR3H comprises SEQ ID NO: 51.
2. An isolated antibody or antigen-binding fragment thereof that specifically interacts and shows measurable affinity to a polypeptide comprising the active portion of PDGF-C (SEQ ID NO: 2) or a polypeptide having at least 90% sequence identity thereto, wherein said antibody comprises a light and a heavy chain, wherein the light chain and the heavy chain of the antibody comprise the respective sequences of (i) SEQ ID NOs: 4 and 10, (ii) SEQ ID NOs: 8 and 14, or (iii) SEQ ID NOs: 9 and 15.