Polypeptide variants
Detergent compositions including polypeptide variants and methods of cleaning and/or treatment of surfaces using such compositions, and fabric treatment compositions including polypeptide variants. The compositions may include surfactants: anionic, nonionic and/or cationic.
1. A detergent composition, comprising: a) a variant comprising one or more substitution(s) compared to SEQ ID NO: 1, wherein the substitution is selected from T1I or T1V, wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 of at least about 90% and the variant has DNase activity; and
b) from about 0.01 to about 99.9 wt % of an adjunct.
2. The detergent composition according to claim 1 , wherein the variant has an improved stability, measured as half-life improvement factor, HIF, compared to the polypeptide shown in SEQ ID NO: 1.
3. The detergent composition according to claim 1 , wherein the variant has at least about 91 but less than about 100% sequence identity to the polypeptide shown in SEQ ID NO: 1.
4. The detergent composition according to claim 1 , wherein the total number of alterations compared to SEQ ID NO: 1 is from about 1 to about 10 alterations.
5. The detergent composition according to claim 1 , wherein the adjunct comprises an additional enzyme comprising a protease, an amylase, a lipase, a cutinase, a cellulase, an endoglucanase, a xyloglucanase, a pectinase, a pectin lyase, a xanthase, a peroxidase, a haloperoxygenase, a catalase, a mannanase, or a combination thereof.
6. The detergent composition according to claim 1 , comprising from about 5 to about 70 wt % surfactant comprising: an anionic surfactant, a nonionic surfactant, or the anionic surfactant and the nonionic surfactant.
7. The detergent composition according to claim 1 , wherein the detergent composition is a laundry detergent composition.
8. The detergent composition according to claim 1 , wherein the detergent composition is a hard surface cleaner.
9. The detergent composition according to claim 8 , wherein the detergent composition is a dish-washing composition.
10. The detergent composition according to claim 1 , comprising (i) from about 2 to about 50 wt % quaternary ammonium ester fabric softener compound selected from compounds of the following formula:
{R 2 (4-m) —N+—[X—Y—R 1 ] m }A-
wherein:
m is 1, 2 or 3 with proviso that the value of each m is identical;
each R 1 is independently hydrocarbyl, or branched hydrocarbyl group;
each R 2 is independently a C 1 -C 3 alkyl or hydroxyalkyl group;
each X is independently (CH 2 ) n , CH2-CH(CH 3 )— or CH—(CH 3 )—CH 2 — and
each n is independently 1, 2, 3 or 4;
each Y is independently —O—(O)C— or —C(O)—O—;
A- is independently selected from the group consisting of chloride, methyl sulfate, and ethyl sulfate;
with the proviso that when Y is —O—(O)C—, the sum of carbons in each R 1 is from about 13 to about 21.