Methods for treating chondrosarcoma using microrna(miR)
This invention is directed to, inter alia, compositions and methods for restoring normal microRNA (miR) expression in chondrosarcoma cells as well as methods for treating and diagnosing chondrosarcoma in individuals in need thereof.
1. A method for treating chondrosarcoma in an individual in need thereof, the method comprising identifying an individual diagnosed with chondrosarcoma or a predisposition thereto and administering to the individual a therapeutically effective amount of one or more chondrosarcoma-inhibiting microRNA (miR), wherein the miR comprises miR-34a, wherein said method results in decreased expression of vascular endothelial growth factor (VEGF), synovial sarcoma X-1 (SSX1), and tyrosine-protein kinase Met (Met), and wherein said method results in decreased chondrosarcoma cellular proliferation, invasion, or metastasis.
2. The method of claim 1 , further comprising administering a chemotherapeutic to the individual.
3. The method of claim 2 , wherein said chemotherapeutic is AMD3100.
4. The method of claim 1 , further comprising administering an inhibitor of miR-181a.
5. The method of claim 1 , wherein the individual is diagnosed with conventional chondrosarcoma, periosteal chondrosarcoma, mesenchymal chondrosarcoma, dedifferentiated chondrosarcoma, clear-cell chondrosarcoma, or extraskeletal myxoid chondrosarcoma.
6. The method of claim 1 , wherein the method further comprises administration of one or more additional anti-cancer therapies to the individual.
7. The method of claim 6 , wherein said one or more additional anti-cancer therapies is surgical ablation of the chondrosarcoma.
8. The method of claim 1 , wherein the miR is administered by a nanopiece comprising a length of about 100 nm and width/diameter of about 20 nm.
9. The method of claim 1 , wherein the chondrosarcoma-inhibiting microRNA (miR) is administered to a tumor bed of the chondrosarcoma in the individual following surgical resection of a tumor.