Cell modulation
The present disclosure provides molecules which modulate cell growth. These molecules include those that bind sialic acid which may find application in the treatment and/or prevention of cell proliferation and/or differentiation disorders, cancer and/or it's migration and/or spread.
1. A method of treating cancer, said method comprising administering a sialic acid binding molecule to a subject in need thereof, wherein the sialic acid binding molecule is a multimeric carbohydrate binding module (CBM).
2. The method of claim 1 , wherein the cancer is lung cancer, cervical cancer, colon cancer and/or breast cancer.
3. The method of claim 1 , wherein the multimeric carbohydrate binding module comprises one or more family 40 carbohydrate binding module(s).
4. The method of claim 1 , wherein the multimeric carbohydrate binding module comprises a sialic acid binding domain of Vibrio cholerae NanH sialidase and/or a sialic acid binding domain of Streptococcus pneumoniae NanA sialidase.
5. The method of claim 4 , wherein the Vibrio cholerae NanH sialidase comprises the amino acid sequence of SEQ ID NO: 1 or 2.
6. The method of claim 4 , wherein the Streptococcus pneumoniae NanA sialidase comprises the amino acid sequence of SEQ ID NO: 3 or 4.
7. The method of claim 1 , wherein the multimeric CBM comprises two or more sialic acid binding domains of Vibrio cholerae NanH sialidases and/or two or more sialic acid binding domains of Streptococcus pneumoniae NanA sialidases.
8. The method of claim 1 , wherein the multimeric CBM comprises 2, 3, 4, 5, 6, 7, 8, 9 or 10 sialic acid binding domains of Vibrio cholerae NanH sialidases and/or Streptococcus pneumoniae NanA sialidases.
9. The method of claim 1 , wherein the multimeric CBM is a combination of four sialic acid binding domains of Vibrio cholerae NanH sialidase (Vc4CBM), a combination of two sialic acid binding domains of Vibrio cholerae NanH sialidase and a trimerisation domain (Vc2CBMTD), and/or a combination of two sialic acid binding domains of Streptococcus pneumoniae NanA sialidase and a trimerisation domain (Sp2CBMTD).