Shaking culture apparatus and culture method using the same

1. A shaking culture apparatus comprising:

a culture bag made from a packaging material;

an outer shell container being arranged to house the entirety of said culture bag to be able to perform adjustment of a temperature thereof;

a shaking device comprising a base portion and a frame portion surrounding the outer shell container, and a power source for shaking said outer shell container housing said culture bag;

a detector configured to detect a height of a wave of contents of the culture bag; and

a control panel for adjusting a rotation number of shaking of said outer shell container housing said culture bag so that the height of the wave of the contents becomes constant,

wherein said outer shell container is provided with a container body having a side wall portion and a bottom wall portion, and a lid portion for closing an upper opening of the side wall portion, the side wall portion has a window which is provided as a slit notch in a vertical direction, the bottom wall portion and the lid portion have holes through which tubes are passed,

an internal space of said outer shell container has a cylindrical shape, a circle of a cylinder is positioned on a bottom face of said outer shell container, and a ratio of a diameter of the circle and a height of the cylinder is 1:2 to 2:1,

said culture bag is a gusset bag of a square box shape which is sealed by arranging one pair of films with a gusset between another pair of films and providing a sealing portion at peripheral edges of both of the pair of films,

said culture bag is provided with a spout which is fixed to the culture bag by sealing a base of the spout on a back side of one of the pair of films or one of the another pair of films,

said tubes are provided on an upper surface and a lower surface of said culture bag respectively and each tube is passed through said holes of the bottom wall portion and the lid portion,

contents containing liquid and gas are housed in said culture bag, a volumetric ratio of the liquid and the gas is 1:9 to 9:1, and said culture bag is firmly attached to said outer shell container in the range that said culture bag houses the liquid so that said culture bag is supported by said outer shell container,

said contents in said culture bag can be stirred and mixed by shaking said outer shell container housing said culture bag,

a center of the outer shell container rotates on an orbit of a circular shape,

a rotation direction includes both a horizontal component and a vertical component, and

a magnitude of the vertical component is smaller than a magnitude of the horizontal component provided that the magnitude of the vertical component is not 0,

said outer shell container housing said culture bag has a rotation number of shaking of 10 to 80 rpm and an amplitude of 10 to 30 mm,

the shaking device is loaded with a counter balance, and

wherein the control panel is configured to change a rotation speed and a rotation direction of said shaking device, based on the detected height of the wave, not only stirring of the liquid in a horizontal direction, but also stirring in a vertical direction are realized.

2. The shaking culture apparatus according to claim 1 , wherein said outer shell container comprises a jacket structure through which circulating water can be passed or a rubber heater.

3. The shaking culture apparatus according to claim 1 , wherein the shaking culture apparatus is provided with a sensor for measuring a pH or a dissolved oxygen concentration (DO) of the contents in said culture bag.

4. The shaking culture apparatus according to claim 1 , wherein said culture bag is provided with an injection port for pouring an acid or an alkali to make a pH of said contents constant.

5. The shaking culture apparatus according to claim 1 , wherein said culture bag is connected to each tube for adding and taking out said contents.

6. The shaking culture apparatus according to claim 1 , wherein said culture bag is provided with a tube for supplying air or oxygen to said contents.

7. A culture method comprising a step of culturing an organism or a cell by using the shaking culture apparatus according to claim 1 .