Polymers including galactose based blocks and uses thereof
Block copolymers that include one or more galactose based blocks and one or more cationic blocks; polyplexes including disclosed block copolymers and one or more nucleic acids; and methods of delivering a nucleic acid to a cell which can include delivering a polyplex to the cell.
1. A block copolymer comprising:
one or more N-acetyl-D-galactosamine (GNA) based blocks; and
one or more cationic blocks.
2. The block copolymer according to claim 1 , wherein the one or more cationic blocks comprise aminoethylmethacrylamide (AEMA), 3-guanidinopropyl methacrylamide (GPMA), or combinations thereof.
3. The block copolymer according to claim 1 , wherein the one or more N-acetyl-D-galactosamine (GNA) based block comprises methacrylamido N-acetyl-D-galactosamine (MAGalNAc).
4. The block copolymer according to claim 1 , wherein there are at least 10 repeating units of the cationic block.
5. The block copolymer according to claim 1 , wherein there are at least 30 repeating units of the cationic block.
6. The block copolymer according to claim 1 , wherein there are at least 40 repeating units of the N-acetyl-D-galactosamine (GNA) based block.
7. The block copolymer according to claim 1 , wherein there are at least 60 repeating units of the N-acetyl-D-galactosamine (GNA) based block.
8. A polyplex comprising:
a block copolymer comprising:
one or more N-acetyl-D-galactosamine (GNA) based blocks; and
one or more cationic blocks; and
one or more nucleic acids.
9. The polyplex according to claim 8 , wherein the one or more cationic blocks comprise aminoethylmethacrylamide (AEMA), 3-guanidinopropyl methacrylamide (GPMA), or combinations thereof.
10. The polyplex according to claim 8 , wherein the one or more N-acetyl-D-galactosamine (GNA) based block comprises methacrylamido N-acetyl-D-galactosamine (MAGalNAc).
11. The polyplex according to claim 8 , wherein there are at least 10 repeating units of the cationic block.
12. The polyplex according to claim 8 , wherein there are at least 40 repeating units of the N-acetyl-D-galactosamine (GNA) based block.
13. A method of delivering a nucleic acid to a cell, the method comprising:
delivering a polyplex to a cell, the polyplex comprising:
a block copolymer comprising:
one or more N-acetyl-D-galactosamine (GNA) based blocks; and
one or more cationic blocks; and
one or more nucleic acids.
14. The method according to claim 13 , wherein the one or more cationic blocks comprise aminoethylmethacrylamide (AEMA), 3-guanidinopropyl methacrylamide (GPMA), or combinations thereof.
15. The method according to claim 13 , wherein the one or more N-acetyl-D-galactosamine (GNA) based block comprises methacrylamido N-acetyl-D-galactosamine (MAGalNAc).
16. The method according to claim 13 , wherein there are at least 10 repeating units of the cationic block.
17. The method according to claim 13 , wherein there are at least 40 repeating units of the N-acetyl-D-galactosamine (GNA) based block.