Methods for screening cereblon modifying compounds
A method of screening a cereblon modifying compound for treating a disease or disorder, comprising: obtaining a sample; determining a first protein level of SALL 4 ; administering the cereblon modifying compound to the sample; determining a second protein level of SALL 4 ; comparing the first level and the second protein level of SALL 4 to determine if the cereblon modifying compound induces degradation of SALL 4 ; and selecting the cereblon modifying compound that does not induce degradation of SALL 4.
1. A method of screening a cereblon modifying compound, comprising:
(a) obtaining a sample containing Sal-like protein 4 (SALL 4 ) and cereblon;
(b) administering the cereblon modifying compound to the sample;
(c) determining an interaction between the SALL 4 and the cerebion after step (b), wherein the interaction between the SALL 4 and the cereblon is determined by determining the interaction between amino acid residues 405 - 432 of the SALL 4 protein (SEQ ID NO: 3) and the cereblon or by determining the interaction between amino acid residues 410 - 432 of the SALL 4 protein (SEQ ID NO: 11 ) and the cereblon; and
(d) selecting the cereblon modifying compound that does not induce the interaction between the SALL 4 and the cereblon as determined in step (c).
2. The method of claim 1 , further comprising
(i) determining a first protein level of SALL 4 in the sample prior to the administration of the cereblon modifying compound;
(ii) determining a second protein level of SALL 4 in the sample after the administration of the cereblon modifying compound;
(iii) comparing the first protein level and the second protein level of SALL 4 to determine if the cereblon modifying compound induces degradation of SALL 4 ; and
(iv) selecting the cereblon modifying compound that does not induce the degradation of SALL 4 as determined in step (iii).