Post mortem reconstitution of circulation
View Patent ↗A system for reconstituting circulation in a cadaver includes at least a pump, a fluid driven by the pump, and operative fluid connections between the pump and the cadaver. The system may be used to occlude one or more arterioles within the cadaver. The system may include a first circuit that creates a first fluid path with vessels of the cadaver, with first circuit having a first fluid conduit, a heater unit, a first pump, and a resistance device. The system may also include a second circuit that creates a second fluid path with vessels of the cadaver that are different from the vessels of the first circuit, with the second circuit having a second fluid conduit and a second pump. Alternately, the system may include a first circuit that creates a first fluid path with vessels of the cadaver and a second flow path that creates a second fluid path with the reservoir, where the first circuit includes a first conduit, a second connector, a pump, a reservoir, and a connector.
1. A method of inhibiting fluid loss through endothelium into tissue of a cadaver, the method comprising:
occluding a plurality of small blood vessels of the cadaver;
introducing a first fluid to a first blood vessel of the cadaver, the first fluid being formulated to occlude the plurality of small blood vessels;
causing the first fluid to flow through the first blood vessel towards the plurality of small blood vessels; and
draining the first fluid from the first blood vessel and introducing a second fluid to the first blood vessel, the second fluid being formulated to simulate blood so as to provide a realistic model of a live patient.
2. The method of claim 1 , wherein each of the plurality of small vessels is one of an arteriole, a venule, or a capillary.
3. A method of inhibiting fluid loss through endothelium into tissue of a cadaver, the method comprising:
occluding a plurality of small blood vessels of the cadaver;
introducing a first fluid to a first blood vessel of the cadaver, the first fluid being formulated to occlude the plurality of small blood vessels;
causing the first fluid to flow through the first blood vessel towards the plurality of small blood vessels; and
generating a low-pressure environment within an interior volume of a reservoir, the reservoir being in fluid communication with the first blood vessel through a first inlet of the reservoir so as to cause the first fluid to be drawn away from the first blood vessel.
4. The method of claim 3 , wherein the first fluid is introduced into the first blood vessel at a first location, the first location being displaced from the plurality of small blood vessels.
5. The method of claim 4 , wherein the first blood vessel is a carotid artery or a jugular vein.
6. The method of claim 3 , wherein the reservoir comprises a second inlet, the second inlet being configured to provide surgical suction.
7. A method of isolating a vascular branch from tissue associated with the vascular branch, the method comprising:
perfusing the vascular branch with a first fluid, the first fluid being configured to occlude a plurality of small blood vessels extending from the vascular branch;
introducing the first fluid into the vascular branch at a first location;
causing the first fluid to flow through the vascular branch towards a second location; and
drawing the first fluid from the vascular branch at the second location,
wherein the second location is displaced from the first location, and
wherein at least some of the plurality of small blood vessels are positioned between the first and second locations.
8. The method of claim 7 , wherein each of the small blood vessels has a diameter of less than 500 microns.
9. The method of claim 8 , wherein each small blood vessel extending from the vascular branch is occluded.
10. The method of claim 9 , wherein the vascular branch comprises an aorta or a vena cava.
11. The method of claim 7 , further comprising:
generating a low-pressure environment within an interior volume of a reservoir the reservoir being in fluid communication with the vascular branch through a first inlet of the reservoir so as to cause the first fluid to be drawn from the vascular branch at the second location,
wherein drawing the first fluid from the vascular branch at the second location causes the first fluid to flow through the vascular branch.
12. The method of claim 11 , wherein the reservoir comprises a second inlet, the second inlet being configured to provide surgical suction.