Cell culture substrate
The present invention is to provide a cell culture substrate including a block polymer including a segment having a lower critical solution temperature and a hydrophobic segment, in which the segment having a lower critical solution temperature has a degree of polymerization of 400 to 10,000. Also provided is a cell culture substrate, in which the hydrophobic segment is obtainable by polymerizing a monomer having a particular structure. Also provided is a cell culture substrate being laminated on a supporting medium. Furthermore, a cell culture substrate having an average film thickness of 1,000 nm or less is provided.
1. A cell culture substrate comprising a block polymer comprising a segment having a lower critical solution temperature and a hydrophobic segment,
wherein the segment having a lower critical solution temperature has a degree of polymerization of 400 to 10,000.
2. The cell culture substrate according to claim 1 , wherein the hydrophobic segment is obtainable by polymerizing a monomer represented by the following Formula (1):
wherein R 1 represents a hydrogen atom or a methyl group; and R 2 represents any one of a phenyl group, a carboxyalkyl group having an alkyl with 1 to 8 carbon atoms, a carboxyaralkyl group having an aralkyl with 7 or 8 carbon atoms, a group represented by the following Formula (2), or a group represented by the following Formula (3):
wherein n represents 2 or 3; and R 3 represents an alkyl group having 1 to 3 carbon atoms,
[Chem. 3]
wherein R 4 and R 5 each independently represent a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; and the total number of carbon atoms of R 4 and R 5 is 5 or more.
3. The cell culture substrate according to claim 1 , which is laminated on a supporting medium.
4. The cell culture substrate according to claim 3 , which has an average film thickness of 1,000 nm or less.
5. A cell culture equipment comprising a supporting medium and the cell culture substrate according to claim 1 .
6. The cell culture substrate according to claim 2 , which is laminated on a supporting medium.
7. The cell culture substrate according to claim 6 , which has an average film thickness of 1,000 nm or less.
8. A cell culture equipment comprising a supporting medium and the cell culture substrate according to claim 2 .
9. A cell culture equipment comprising a supporting medium and the cell culture substrate according to claim 3 .
10. A cell culture equipment comprising a supporting medium and the cell culture substrate according to claim 4 .